敷胸方不同剂型的体外透皮吸收效果比较

目的：评价超滤工艺对注射用芪红脉通抗急性心肌缺血和被动皮肤过敏反应的影响。方法：选择舌静脉注射垂体后叶素（0.75 U·kg^-1）构建急性心肌缺血大鼠模型，以心电图ST段变化和血清超氧化物歧化酶（SOD），丙二醛（MDA）的含量变化为药效学评价指标，比较注射用芪红脉通超滤前后对垂体后叶素致大鼠急性心肌缺血的影响，以大鼠被动皮肤过敏试验考察该制剂的安全性。结果：超滤前后注射用芪红脉通在成分上无明显变化；与模型组相比，超滤前后样品均能显著降低心电图ST段的抬升，超滤前中剂量及超滤后高剂量样品组均能显著提高血清SOD活性，各给药组均能显著降低血清MDA的产生，但超滤前后这些指标均无显著差异；超滤后样本的被动皮肤过敏呈阴性。结论：注射用芪红脉通具有显著的抗急性心肌缺血作用，超滤工艺提高了制剂的安全性，对药效无影响。

Comparison of in vitro Percutaneous Absorption Effectiveness Between Different Formulations of Fuxiong Prescription

Objective: To evaluate effects of ultrafiltration process on against acute myocardial ischemia and passive cutaneous anaphylaxis of Qihong Maitong injections(QM). Method: Rat model of acute myocardial ischemia was established by vena lingualis injections of pituitrin(0.75 U·kg^-1),taking electrocardiogram ST segment changes,serum superoxide dismutase(SOD) activities and malonaldehyde(MDA) levels in blood serum as indexes,effects of QM(before and after been ultrofiltraed) on protection against pituitrin-induced myocardial ischemia in rats,passive cutaneous anaphylaxis of samples was tested to explore safety of QM. Result: No obvious changes were obsovered in compositions after ultrafiltration;compared with model group,both of ultrofiltraed and un-ultrofiltraed samples could significantly reduce lifting of electrocardiogram ST segment,the middle dose group before ultrafiltration and the high dose group after ultrafiltration could significantly increase serum SOD activities,every dose group could significantly reduce production of serum MDA levels,but no significant changes were obsoverd between groups administrated with ultrofiltraed and un-ultrofiltraed samples.Passive cutaneous anaphylaxis of ultrafiltratded samples were negative. Conclusion: QM showed significant activties of against acute myocardial ischemia.Ultrafiltration process improved security of QM,but it had not influenced their activities of anti-myocardial ischemia.