铁皮石斛对运动疲劳小鼠能量代谢和CNTFmRNA表达的影响

目的：通过建立T淋巴细胞（T细胞）免疫功能缺陷的小鼠动物模型，观察壮尔颗粒对小鼠体内细胞因子平衡状态的影响。方法：将48只小鼠随机分为空白组，模型组，玉屏风颗粒组，壮尔颗粒高、中、低剂量组，空白组和模型组ig给予生理盐水，其余各组分别ig给予按玉屏风颗粒溶液（3.38 g·kg^-1），壮尔颗粒高、中、低剂量组（4.04，2.02，1.01 g·kg^-1）。给药10 d后，空白组经鼻滴入10 μL无菌生理盐水，其余各组经鼻滴入10 μL肺炎链球菌悬液（密度为1×10⁵CFU/mL），制备小鼠T细胞免疫功能缺陷的动物模型。应用流式微球技术（CBA）分别测定小鼠血清和小鼠脾脏白介素（IL）-2等细胞因子的水平，免疫组织化学染色法检测小鼠脾脏中IL-2和IL-4的表达。结果：与正常组比较，模型组小鼠血清和脾脏中IL-2，肿瘤坏死因子（TNF），干扰素-γ（IFN-γ）的水平均有显著性下降（P<0.05），同时IL-4，IL-6，IL-10，IL-17A的水平有显著性升高（P<0.05）。与模型组比较，壮尔颗粒高、中剂量组可显著提高小鼠血清和脾脏中IL-2，TNF，IFN-γ水平（P<0.05），同时IL-4，IL-6，IL-10，IL-17A的水平均有显著性下降（P<0.05），并且小鼠脾脏IL-2表达上调和IL-4表达下调，有统计学意义（P<0.05）。结论：壮尔颗粒能够通过调节小鼠体内细胞因子的平衡来增强机体免疫功能。

Effects of Dendrobium officinale on Energy Metabolism and Expression of CNTF mRNA in Athletic Fatigue Mice

Objective: To investigate the effects of Dendrobium officinale on energy metabolism and expression of ciliary neurotrophic factor (CNTF) mRNA in athletic fatigue mice. Method: Sixty male Kunming mice were divided randomly into control group, D. officinale low-dose group,middle-dose group,high-dose group. The low-dose group,middle-dose group and high-dose group were intragastricaly (ig)administrated with D. officinale respectively according to 0.75,1.5,4.5 g·kg^-1 while control group was ig given same volume normal saline, once a day for 30 days. The weight carrying swimming test were used to observe exhaustive weight carrying swimming time and to examine levels of serum urea, muscle glycogen, hepatic glycogen,lactic acid and the expression of CNTF mRNA. Result: Compared to control group, exhaustive weight carrying swimming time in low-dose group increased (P<0.05),and in both middle-dose group and high-dose group increased significantly(P<0.01). Compared to control group, the levels of serum urea and lactic acid in both low-dose group and middle-dose group decreased(P<0.05),and in high-dose group decreased significantly(P<0.01). Compared to control group, the levels of muscle glycogen and hepatic glycogen in middle-dose group increased (P<0.05)and in high-dose group increased significantly (P<0.01). Compared to control group, the expression of CNTF mRNA in low-dose group increased (P<0.05), and in middle-dose group and high-dose group increased significantly(P<0.01). Conclusion: D. officinale could increase glycogen store,decrease the level of serum urea and lactic acid accumulation,meantime, could up-regulate the expression of CNTF mRNA, which may be one of mechanisms that D.officinale alleviate athletic fatigue by regulating energy metabolism and nourishing muscle.