虎杖中白藜芦醇诱导肝癌细胞HepG-2凋亡及其对Bcl-2和Bax蛋白表达的影响

目的：观察爱罗咳喘宁口服液对慢性阻塞性肺疾病（COPD）大鼠模型血浆及支气管肺泡灌洗液（BALF）中白三烯B4（LTB4）、白细胞介素-6（IL-6）及肺组织病理形态的影响。方法：采用脂多糖（LPS）加烟雾诱导COPD大鼠模型，大鼠随机被分为正常组、模型组、爱罗咳喘宁口服液低、中、高剂量组。正常组、模型组灌胃蒸馏水（10 mL·kg^-1·d^-1），爱罗咳喘宁口服液低、中、高剂量组分别灌胃混悬液（5，10，20 g·kg^-1·d^-1），连续7 d。酶联免疫法（ELISA）测定各组大鼠血浆和BALF 中LTB4，IL-6的含量。结果：与正常组相比，模型组大鼠血浆和 BALF 中LTB4，IL-6水平均显著高于正常组（均P<0.05），与模型组相比，爱罗咳喘宁口服液低、中、高剂量组LTB4和IL-6含量均显著降低（均P<0.05）。结论：爱罗咳喘宁口服液治疗COPD的作用机制可能与抑制LTB4，IL-6的释放有关。

Apoptosis of HepG-2 Cell Induced by Resveratrol and Its Effects on Expressions of Proteins Bcl-2 and Bax

Objective: To study the effect of Ailuo Kechuanning oral liquid on leukotriene B4(LTB4), interleukin-6 (IL-6)levels and pathological morphology in plasma and bronchial alveolar lavage fluid (BALF) of rats with chronic obstructive pulmonary disease(COPD). Method: Lipopolysaccharide (LPS) combined with smoke was used to establish COPD rat model;the experimental animals were randomly divided into normal control group,model group,Ailuo Kechuanning oral liquid groups (5,10,20 g·kg^-1·d^-1), enzyme-linked immunosorbent assay(ELISA) was used to determine the change of LTB4, IL-6 levels in rat plasma and BALF in the rats of all groups before and after treatment. Result: The levels of LTB4,IL-6 in plasma and BALF of the model group were significantly higher than those in normal control group;compared with the model group,the levels of LTB4,IL-6 in plasma and BALF of the rats treated with Ailuo Kechuanning oral liquid were significantly decreased. Conclusion: The mechanism of Ailuo Kechuanning oral liquid for treatment of COPD may be related to the inhibition of LTB4, IL-6 release.