VEGF对大鼠海马回神经干细胞体外增殖和分化的影响

目的： 观察血管内皮细胞生长因子(VEGF)对大鼠海马回神经干细胞（NSCs）增殖和分化的影响。方法: 自生后第3 d的SD大鼠脑海马区分离NSCs，进行体外培养并传代，分为2组：（1）VEGF组加入150 μg/L VEGF的培养液培养；（2）对照组未加VEGF。培养7 d后，撤除VEGF，分别在第7 d及第11 d通过相差显微镜观察细胞形态，并用免疫荧光方法检测nestin和NF的表达，计算各组免疫染色阳性细胞率。结果: 在VEGF作用下第7 d，VEGF组nestin阳性细胞率为52.19%±7.95%，多于对照组的29.26%±4.12%（P<0.01），NF阳性细胞率为22.33%±4.13%，对照组仅有38.62%±5.31%（P<0.01）；在实验第11 d，VEGF组NF阳性细胞率为43.10%±3.70%，对照组仅30.56%±4.16%，P<0.01。结论: 本实验提示VEGF能促进NSCs的增殖,并抑制其分化。

Effect of VEGF on proliferation and differentiation of neural stem cells from the hippocampal gyrus of rat in vitro

AIM: To observe the effects of vascular endothelial growth factor (VEGF) on the proliferation and differentiation of neural stem cells (NSCs) of rats in vitro.METHODS: NSCs isolated from the hippocampal gyrus of SD rats were primary cultured and subcultured,and then divided into two groups: (1) the cells in VEGF group were treated with 150 μg/L VEGF in the culture system,and VEGF was removed at the 7 th day;(2) control group (without VEGF treatment).The cellular morphology of two groups was observed by contrast phase microscope.Nestin and NF-200 expressing cells were detected via immunofluorescence method.The percentages of the immunostaining positive cells in each group at the 7 th day and at the 11 th day were determined.RESULTS: At the 7 th day,the percentage of nestin positive cells in VEGF group was 52.19%±7.95%,vs 29.26%±4.12% in control group (P<0.01).The percentage of NF positive cells in VEGF group was 22.33%±4.13%,vs 38.62%±5.31% in control group (P<0.01).At the 3 th day after VEGF was removed,the percentage of NF positive cells in VEGF group was 43.10%±3.70%,vs 30.56%±4.16% in control group (P<0.01).CONCLUSION: VEGF stimulates the proliferation of neural stem cells and inhibits their differentiation.