高脂饮食诱导的肥胖大鼠体内瘦素的变化

目的 高脂饮食诱导的肥胖大鼬肥胖特点及体内瘦素的变化.方法 利用含20%猪油的高脂饲料和普通饲料分别喂养SD大鼠20只和10只8周,建立饮食性肥胖的动物模型.用酶联免疫法测定两组大鼠血清中瘦素含量,然后对肥胖组与对照组的体重、肝、肾、脑组织的重量、胆固醇、甘油三酯、瘦素（leptin）含量进行比较.结果 肥胖组大鼠体重、脑、肝、肾组织重量均＞对照组,且肥胖组大鼠脑、肝、肾重量与体重呈显著正相关,p＜0.01.肥胖组大鼠血清甘油三酯、瘦素含量明显高于对照组,p＜0.05.结论 含20%高脂饲料能够诱导部分雄性SD大鼠发展为饮食性肥胖.在肥胖组大鼠体内存在着高瘦素血症,提示有瘦素抵抗.

The Change of Leptin in Rat Obesity Induced by Lipid Rich Food

Objective To study the character of rat obesity and change of leptin induced by high fat diet. Methods 20 SD rats and 10 SD rats were feed with lipid rich food (20% lard) and normal food for 8 weeks to make alimentary obesity model respectively. The level of serum leptin in two group were estimated with enzyme-linked immuno-sorbent assay. The level of rat body weight , liver, kidney, weight of brain tissue ,cholesterol, triglyceride and leptin in two groups were compared respectively. Results The level of rat body weight, brain, liver and kidney in obesity group were higher than that of in contrast group and the weight of brain, liver and kidney is significant positive correlation with body weight in obesity group (p<0.01). The level of serum triglyceride and leptin in obesity group were higher than that of in contrast group(p<0.05) . Conclusion It can induce alimentary obesity in part of male SD rats can be induced with continuous lipid rich food including 20% lard. High concentration of serum leptin was existed in obesity group.