| 胃癌单克隆抗体-羟基喜树碱免疫偶合物的制备及其细胞毒特征 |
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| 引用本文： | 涂水平,吴裕炘,曹伟丽,章永平,华家柽,田盛海.胃癌单克隆抗体-羟基喜树碱免疫偶合物的制备及其细胞毒特征[J].现代免疫学,1993(2). |
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| 作者姓名： | 涂水平 吴裕炘 曹伟丽 章永平 华家柽 田盛海 |
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| 作者单位： | 上海第二医科大学瑞金医院内科，上海第二医科大学瑞金医院内科，上海第二医科大学瑞金医院内科，上海第二医科大学瑞金医院内科，中科院上海药物研究所，中科院上海药物研究所 上海 200025，上海 200025，上海 200025，上海 200025 |
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| 摘 要： | 采用共价交联法将胃癌单克隆抗体RSF_9与羟基喜树碱进行交联,交联物中每分子抗体可引入13±4.2分子的药物,而抗体活性保持85％以上。体外细胞毒试验显示交联物RSF_9-HCPT对胃癌细胞的杀伤是游离药物的4.7倍,而对非靶细胞的杀伤只有游离药物的1/11,表明交联物对胃癌靶细胞具较强的选择性杀伤作用。
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| 关 键 词： | 胃癌 单克隆抗体 羟基喜树碱 免疫偶合物 细胞毒 |
PREPARATION OF HYDROXYCAMPTOTHECINE-MONOCLONAL ANTIBODY RSF_9 IMMUNOCONJUGATE AND CHARACTERIZATION OF ITS CYTOTOXICITY TO GASTRIC CANCER CELLS IN VITRO |
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| Tu Shuipin Wu Yuixin Chou Weili Zhang Yongpin.PREPARATION OF HYDROXYCAMPTOTHECINE-MONOCLONAL ANTIBODY RSF_9 IMMUNOCONJUGATE AND CHARACTERIZATION OF ITS CYTOTOXICITY TO GASTRIC CANCER CELLS IN VITRO[J].Current Immunology,1993(2). |
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| Authors: | Tu Shuipin Wu Yuixin Chou Weili Zhang Yongpin |
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| Institution: | Tu Shuipin Wu Yuixin Chou Weili Zhang Yongpin Department of Digestion,Rui jin Hospital,Shanghai Second Medical UniversityHua Jiasheng Teng Shenghai Shanghai Institute of Materia Medica,Academia Sinica |
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| Abstract: | Monoclonal antibody RSF9 against human gastric cancer was linked convalently to hydroxycamptothecine (HCPT) to form an immuno-conjugate (HCPT-RSF9). The linked molecular ratio of RSF9 to HCPT was 13±4.2. ELISA revealved that the specific immunoreactivity of the McAb was well preserved in the conjugate (over 85%). The cytotoxicity of HCPT-RSF9 to human gastric cancer cells (MKN-28) was determined by MTT method. The results showed that the cytotoxicity of HCPT-RSF9 to gas-trie cancer cells was 4.7 times that of free HCPT, while to non-target cells was only 1/11 of that of free HCPT, Suggesting highly selective cytotoxicity to target cells. |
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| Keywords: | gastric cancer Monocolonal antibody hydroxycamptothecine immune-conjugate cytotoxicity |
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