受体放射分析中标记配体质量的评价

受体的放射性配体结合分析是目前受体研究中常用的方法。其中，标记配体的质量十分重要。标记配体的质量主要有两个方面，即标记配体的纯度（结合力）及其与未标记配体的结合性能的一致性。目前，多数文献报道主雍以受体饱和结合试验的双倒数来测试标记配体的结合力，再以结合力校正标记配体量，得到活性的杯记配体量。在此基础上进行自身转换试验，测定标记配体比放射性及其与未标记配体结合性能的等效性。采用这些方法，作者对＾１

Assessment of Radioligand Quality in Receptor-Binding Assay

Radioligand binding assay is routinely utilized throughout the world to study receptor and their related binding sites. Importance of radioligand quality is tacitly accepted by mostinvestigators. There are two main parameters of radioligand quality. The first is purity of radioligand (bindability) and'the second is it equivalence of radioligand to the native ligand. The currentmethod used by most laboratories to test the quality is titration of the radioligand with increasingconcentration of receptor to determine the maximum bindability by double reciprocal plot and comparison of the ability of radioligand to compete with itself for binding (saturation binding curve)with the ability of native ligand to competitively inhibit binding of the radioligand to determine thespecific radioactivity and the equivalence. This paper reports these methods and their application inassessment of 125 I - hCG.