| 荧光定量检测HBV DNA与乙肝两对半结果对比分析研究 |
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| 引用本文： | 雷学忠,赵建勤,刘丽,丁柳,赵连三. 荧光定量检测HBV DNA与乙肝两对半结果对比分析研究[J]. 四川医学, 2000, 21(11): 949-953 |
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| 作者姓名： | 雷学忠 赵建勤 刘丽 丁柳 赵连三 |
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| 作者单位： | 华西医科大学附属第一医院,610041 |
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| 摘 要： | 目的为了对乙肝患者体内HBV复制及传染性有更直接地了解,亦更有利于对临床HBV感染的诊断、治疗方案的选择及疗效判定.方法运用荧光定量PCR(FQ-PCR)和ELISA两种方法同时检测了310份肝炎患者血清,并对结果进行了对比分析.结果乙肝大三阳患者血清HBV DNA检出率为92.5%(74/80),平均拷贝数为4.10×1010/ml;小三阳患者血清HBV DNA检出率为32.35%(22/68),平均拷贝数为1.31×109/ml;HBsAg(+)、HBcAb(+)组血清HBV DNA检出率为45.71%(32/70),平均考贝数为4.08×108ml;HBV-M全阴性组血清HBV DNA检出率仍达6.67%(2/32),平均拷贝数仍达1.54×108/ml.结论HBV-M阴性患者仍有HBV DNA阳性者,因此对临床HBV感染、复制及传染性的判断以及指导治疗,除乙肝二对半标志物ELISA检测外,加做HBV DNA FQ-PCR检测同样具有重要意义.
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| 关 键 词： | 乙肝二对半 HBV DNA 荧光定量PCR ELISA |
| 修稿时间： | 2000-08-04 |
Quantitative detection of HBV DNA by FQ-PCR and comparative analysis of its results with HBV-M by ELISA |
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| Lei Xuezhong,Zhao Jianqin,Liu Li,et al The First University Hospital of West China University of Medical Sciences,Chengdu. Quantitative detection of HBV DNA by FQ-PCR and comparative analysis of its results with HBV-M by ELISA[J]. Sichuan Medical Journal, 2000, 21(11): 949-953 |
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| Authors: | Lei Xuezhong Zhao Jianqin Liu Li et al The First University Hospital of West China University of Medical Sciences Chengdu |
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| Affiliation: | Lei Xuezhong,Zhao Jianqin,Liu Li,et al The First University Hospital of West China University of Medical Sciences,Chengdu,610041 |
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| Abstract: | |
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| Keywords: | Hepatitis B Virus HBV DNA FQ PCR ELISAx |
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