HBV—DNA定量与HBV血清学标志物的相关性探讨

目的探讨HBV—DNA定量与HBV血清学标志物（HBV—M）的相关性。方法采用实时荧光定量PCR（FQ—PCR）检测449例乙肝感染者血清的HBV—DNA含量，并用酶联免疫吸附试验（ELISA）对其血清学标志物进行检测。结果在不同HBV—M模式中，HBV—DNA与preS1总检出率无显著差异。在模式HBsAg（＋）、HBeAg（＋）和HBcAb（＋）中血清HB—DNA含量明显高于其他模式。在278例HBV—DNA阳性的标本中，HBV—DNA与preS1检出率无显著差异（P〉0．05），HBV—DNA与HBeAg检出率有显著差异（P〈0．01），同时preS1阳性组HBV～DNA定量值显著高于preS1阴性组。结论HBV—DNA或preS1与HBV复制密切相关，preS1较HBeAg更能敏感反映HBV在体内的复制状况，联合检测HBV—DNA与HBV血清学标志物，在乙型肝炎的诊断治疗中更有重要的临床指导价值。

Relationship between the quantification of HBV - DNA and HBV markers

Objective To study the relationship between the quantification of HBV - DNA and HBV markers（ HBV - M）. Methods HBV - DNA levels were tested in 449 hepatitisB patient serum samples by real time fluorescent quantitative PCR（ FQ -PCR） ,and HBV -M were also tested by ELISA. Results In different HBV -M groups the total serum HBV - DNA and the preSl positive rate had no significant difference. In the group with positive HBsAg, HBeAg, HbcAb,the HBV - DNA levels were significantly higher than that of groups. In 278 serum samples with positive HBV - DNA, HBV - DNA and the preS1 positive rate had no significant difference（ P 〉 0. 05 ）, HBV - DNA and the HBeAg positive rate had significant difference（ P 〈0. 01 ）. In the group with positive preS1, the HBV - DNA levels were significantly higher than the groups with negative preS1. Conclusion HBV - DNA or preS1 closely correlates with hepatitis B virus replication. It showed that preSl was more sensitive than HBeAg in diagnosising hepatitis B virus replication, it is necessary to detect HBV - DNA and HBV markersin the diagnosis and therapy of hepatitis B.