白藜芦醇对实验性脑缺血的保护作用

目的探讨不同剂量白藜芦醇对局灶性脑缺血梗死体积和脑水肿的影响及对核因子-KB(NF-kB)p65表达的影响。方法采用大鼠大脑中动脉闭塞模型,用红四氮唑(TTC)染色显示脑梗死范围,脑水肿的测定用干湿重法,使用免疫组织化学技术观察NF-kBp65的表达。结果白藜芦醇剂量为10^-^7、10^-^6g／kg时有效降低脑梗死体积,10^-^7、10^-^6g／kg白藜芦醇能明显减轻脑水肿程度,在10^-^7g／kg浓度时白藜芦醇能抑制NF-kBp65的表达。结论白藜芦醇具有缺血脑保护作用,其作用与NF-kB的抑制有关。

The neuroprotection of resveratrol in the experimental cerebral ischemia

OBJECTIVE: To study the effect of resveratrol on cerebral infarct volume, cerebral edema as well as the expression of nuclear fac for (NF-kappaB) p65 in the experimental permanent middle cerebral artery occlusion. METHODS: A middle cerebral artery occlusion model was constructed in 20 Wistar rats with Longa's method. Then the 20 rats were divided into 4 groups: control group, and 3 resveratrol groups (resveratrol of the concentrations of 10(-8) g/kg, 10(-7) g/kg, and 10(-6) g/kg were injected intravenously) (group 1- 4). The area of infarct was examined by 2,3,5-triphenyltetrazolium chloride (TTC) staining. The water content was calculated by dry and wet weight method. The expression of NF-kappaB p65 was investigated with immunohistochemistry. RESULTS: The ischemic volume was 241 +/- 36, 222 +/- 39, 126 +/- 16, and 128 +/- 19 * in the groups 1 - 4 respectively, and the volume ratio was 0.49 +/- 0.04, 0.47 +/- 0.04, 27 +/- 0.02, and 0.43 +/- 0.02 respectively. The brain water content was 83.5% +/- 1.5%, 82.9% +/- 1.4%, 79.5% +/- 1.5%, and 79.9% +/- 1.4% in groups 1 - 4 with a significant difference between 10(-7) g/kg resveratrol group and pure ischemia group and 10(-6) g/kg resveratrol group and pure ischemia group (both P < 0.05). NF-kappaB p65 was not expressed in the normal control group and sham operation group, and was expressed 2 hours after pure ischemia. The expression of NF-kappaB p65 positive cells was the most significant 12 hours after the pure ischemia and began to decrease 24 hours after ischemia. CONCLUSION: Resveratrol has a neuroprotective effect on the ischemic brain. This effect is related to the inhabitation of expression of NF-kappaB p65.