| Role of E.coli DNA in systemic inflammatory response syndrome |
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| 作者姓名： | 潘文东 周红 郑江 夏培元 秦孝建 鲁永玲 肖光夏 |
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| 作者单位： | Institute of Burn Research，Southwest Hospital，Third Military Medical University，Chongqing 400038，China，Institute of Burn Research，Southwest Hospital，Third Military Medical University，Chongqing 400038，China，Institute of Burn Research，Southwest Hospital，Third Military Medical University，Chongqing 400038，China，Institute of Burn Research，Southwest Hospital，Third Military Medical University，Chongqing 400038，China，Institute of Burn Research，Southwest Hospital，Third Military Medical University，Chongqing 400038，China，Institute of Burn Research，Southwest Hospital，Third Military Medical University，Chongqing 400038，China，Institute of Burn Research，Southwest Hospital，Third Military Medical University，Chongqing 400038，China |
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| 基金项目： | National Natural Science Foundation of China (No. 30070299) |
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| 摘 要： | Objective: To investigate whether bacterial DNA involving in the pathogenesis of systemic inflammatory response syndrome (SIRS) and possible mechanism. Methods: Escherichia coli DNA (EC DNA) was extracted from Escherichia coli 25922 with alkaline lysis method. The mice mortality was observed after EC DNA was injected into mice via caudal vein. The changes of serum TNF-α and IL-6 levels in rats were measured with ELISA after rats were given EC DNA. Calf thymus DNA and lipopolysaccharide (LPS) were used as the control, respectively. Results: EC DNA led mice to death with notable dose-effect relationship (LD50 = 11. 51 mg/kg), but CT DNA didn't. The peak level of TNF-α was lower in EC DNA group than in LPS group (P<0. 05), though the former reaching the peak I h earlier than the latter. However, they had coordinate ability to induce IL-6 release in rats, and no significant difference was seen in serum IL-6 peak level between 2 groups. Conclusion: EC DNA leads mice to death, and induces the increases of s
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| 关 键 词： | 系统性炎性反应综合征 大肠杆菌DNA 作用 TNF－α IL－6 |
Role of E.coli DNA in systemic inflammatory response syndrome |
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| PAN Wen-dong,ZHOU Hong,ZHENG Jiang,XIA Pei-yuan,QIN Xiao-jian,LU Yong-ling,XIAO Guang-xia.Role of E.coli DNA in systemic inflammatory response syndrome[J].Journal of Medical Colleges of PLA(China),2002,17(3):210-213. |
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| Authors: | PAN Wen-dong ZHOU Hong ZHENG Jiang XIA Pei-yuan QIN Xiao-jian LU Yong-ling XIAO Guang-xia |
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| Abstract: | Objective: To investigate whether bacterial DNA involving in the pathogenesis of systemic inflammatory response syndrome (SIRS) and possible mechanism. Methods: Escherichia coli DNA (EC DNA) was extracted from Escherichia coli 25922 with alkaline lysis method. The mice mortality was observed after EC DNA was injected into mice via caudal vein. The changes of serum TNF-α and IL-6 levels in rats were measured with ELISA after rats were given EC DNA. Calf thymus DNA and lipopolysaccharide (LPS) were used as the control, respectively. Results: EC DNA led mice to death with notable dose-effect relationship (LD50=11.51 mg/kg), but CT DNA didn't. The peak level of TNF-αwas lower in EC DNA group than in LPS group (P＜0. 05), though the former reaching the peak I h earlier than the latter. However, they had coordinate ability to induce IL-6 release in rats, and no significant difference was seen in serum IL-6 peak level between 2 groups. Conclusion: EC DNA leads mice to death, and induces the increases of serum TNF-αand IL-6 levels in rats. EC DNA has the effect equal to LPS in inducing SIRS by triggering cytokines cascade. |
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| Keywords: | Escherichia coli DNA systemic inflammatory response syndrome IL-6 |
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