| 肺鳞癌患者癌组织和血液中微卫星DNA变异的检测 |
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| 引用本文： | 张国俊,张岚,高冬玲,陈奎生,张蕾,张云汉. 肺鳞癌患者癌组织和血液中微卫星DNA变异的检测[J]. 郑州大学学报(医学版), 2004, 39(2): 188-191 |
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| 作者姓名： | 张国俊 张岚 高冬玲 陈奎生 张蕾 张云汉 |
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| 作者单位： | 河南省肿瘤病理重点实验室,郑州大学第一附属医院病理科,郑州,450052;郑州大学第一附属医院呼吸科,郑州,450052;河南省肿瘤病理重点实验室,郑州大学第一附属医院病理科,郑州,450052 |
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| 基金项目： | 河南省自然科学基金资助项目 0211040200,“十五”“211工程”重点学科建设项目 教重办(2002)第2号 |
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| 摘 要： | 目的：检测肺鳞癌患者癌组织和外周血中微卫星DNA的变异。方法：选择30例原发性肺鳞癌患者的癌组织、外周血液标本及30例肺部良性疾病患者的外周血液标本,用PCR—银染法,对其中的20个微卫星位点进行检测。结果：癌组织微卫星DNA变异的检出率较高(26％),肺鳞癌患者外周血20个位点微卫星DNA变异的检出率(20．2％)与癌组织相比差异均无统计学意义,检测出的敏感位点有D3S1067、D3S1447、D3S1611、D3S1284、D3S1110、D3S1007、D5S346、D9S1748。30例肺部良性疾病患者外周血在全部20个微卫星DNA位点上没有检测到变异。结论：肺鳞癌患者存在微卫星DNA的变异,进行外周血中敏感位点微卫星DNA变异的检测对肺鳞癌的早期诊断有重大意义。
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| 关 键 词： | 肺肿瘤 鳞癌 微卫星DNA 聚合酶链反应 |
| 修稿时间： | 2003-10-22 |
Investigation of microsatellite DNA variations in cancer tissues and blood of lung squamous cell carcinoma patients |
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| ZHANG Guojun,ZHANG Lan,GAO Dangling,CHEN Kuisheng,ZHANG Lei,ZHANGYunhan Henan Key Laboratory of Tumor Pathology. Investigation of microsatellite DNA variations in cancer tissues and blood of lung squamous cell carcinoma patients[J]. Journal of Zhengzhou University: Med Sci, 2004, 39(2): 188-191 |
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| Authors: | ZHANG Guojun ZHANG Lan GAO Dangling CHEN Kuisheng ZHANG Lei ZHANGYunhan Henan Key Laboratory of Tumor Pathology |
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| Affiliation: | ZHANG Guojun,ZHANG Lan,GAO Dangling,CHEN Kuisheng,ZHANG Lei,ZHANGYunhan Henan Key Laboratory of Tumor Pathology,Department of Pathology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052 Department of Respiratory,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052 |
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| Abstract: | Aim : To investigate the microsatellite DNA variations in cancer tissues and blood samples of lung squamous carcinoma patients and its value to early diagnosis of lung squamous carcinoma. Method: PCR-silver staining method was used to detect microsatellite DNA in cancer tissues and blood from 30 patients with lung squamous carcinoma. Results : The rates of microsatellite DNA variation were higher, and these variations were also easily seen in cancer tissues as well as in blood samples. The sensitive gene sites included D3S1067, D3S1447, D3S1611, D3S1284, D3S1110, D3S1007, D5S346, D9S1748. Conclusion: Microsatellite DNA variations exist in lung squamous carcinoma. Detecting the variations of blood will be meaningful to early diagnosis of lung squamous carcinoma. |
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| Keywords: | lung neoplasm squamous cell carcinoma microsatellite DNA PCR |
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