IM-94对肝癌细胞的杀伤作用的实验研究

目的 探讨IM-94对人肝癌细胞的杀伤作用。方法 采用MTT法分析IM-94对人肝癌细胞772l生长的影响，用流式细胞术检测细胞有丝分裂周期及细胞凋亡，用H．E．染色分析细胞的核分裂相。结果 IM-94对肝癌细胞有明显的抑制作用，从流式细胞术分析细胞阻滞在G2 M期，并诱发细胞凋亡，在24h后，出现凋亡小峰。结论 IM-94能阻滞细胞分裂，诱导肝癌细胞凋亡。

The killing effect of IM-94 on hepatocellular carcinoma cells

OBJECTIVE To explore the killing effect of IM 94 to human hepatocellular carcinoma cells. METHOD MTT method was used to analyse the influence of IM 94 on human hepatocellular carcinoma. Flow cytometry was applied to detected the mitosis cycle and cell apoptosis , and karyoschisis was analysed by HE staining.RESULTS IM 94 has obvious inhibitory effect on hepatocellular carcinoma cells. Cell was blocked at G2+M stages, and cell apoptosis was induced. A small apoptosis spike was appeared in 24 hours.CONCLUSION IM 94 can block mitosis and induce apoptosis of hepatocellular carcinoma cells.