P-选择素在大鼠重症急性胰腺炎时肺损伤中的作用

目的探讨重症急性胰腺炎(severe acute pancreatitis,SAP)并发急性肺损伤(acutelung injure,ALI)之大鼠P-选择素浓度的变化及其机制。方法取20只大鼠,随机分为假手术组(A组)及胰腺炎组(B组),每组10只。胰管内逆行性注射3%牛磺胆酸钠的方法建立SAP并发ALI大鼠模型,24小时后检测血清淀粉酶、P-选择素和肺组织P-选择素浓度,并取胰腺和肺组织做病理形态学检查。结果制模后B组大鼠血清淀粉酶、P-选择素和肺组织P-选择素浓度较对照组显著升高(P<0.05);A组胸、腹腔内及胰腺、肺组织均无明显病理变化。B组腹水形成,腹腔内组织广泛分布有皂化斑,胰腺广泛水肿、出血、坏死,胸腔内有胸水形成,肺组织存在组织结构紊乱,灶性或片状肺不张,肺泡腔及间质出血等病理学改变,胰腺及肺组织均发现有大量的中性粒细胞浸润。结论SAP合并ALI时,血清P-选择素和肺组织P-选择素浓度均同时异常升高,提示它们在其发病机制中可能具有重要作用。

Experimental Study of P-selectin of Rats with Sever Acute Pancreatitis with Lung Injury

Objective To study of P-selectin of rats with Severe Acute Pancreatitis with Lung Injury.Methods 20 healthy Wistar rats were randomly divided into two groups:normal group(A group)and SAP group(B group).The model of severe acute pancreatitis was induced by retrograde injection of 3% sodium taurocholate into bilio-pancreatic duct in group B.All rats were sacrificed,and blood was drawn and collected from portal vein after SAP had been produced fro 24 hours.The concentration of serum amylase,P-selectin and pulmonary tissue P-selectin were measured.Pathologic alteration of pancreas and lung were observed under microscope.Results The concentration of serum amylase,P-selectin and pulmonary tissue P-selectin were lower in normal rats than that in SAP rats(P<0.05).Conclusion P-selectin plays an important role in rats with severe acute pancreatitis with lung injury.