| 昆明山海棠总生物碱对人结肠癌HCT116细胞增殖和凋亡的影响 |
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| 引用本文： | 黄晓春,刘晋档,周燕虹,敖琳,杨录军,曹佳.昆明山海棠总生物碱对人结肠癌HCT116细胞增殖和凋亡的影响[J].第三军医大学学报,2009,31(22):2246-2248. |
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| 作者姓名： | 黄晓春 刘晋档 周燕虹 敖琳 杨录军 曹佳 |
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| 作者单位： | 第三军医大学军事预防医学院军事毒理学教研室,重庆,400038 |
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| 摘 要： | 目的 研究昆明山海棠总生物碱(total alkaloids of tripterygium hypoglaucum hutch,THHta)对人结肠癌HCT116细胞增殖及凋亡的影响.方法 采用MTT法检测THHta对HCT116细胞增殖的抑制作用;用倒置及荧光显微镜观察THHta作用后HCT116细胞形态的变化;流式细胞术检测THHta对HCT116细胞周期及凋亡的影响,Western bolt检测凋亡相关caspase-3及PARP蛋白表达.结果 THHLa能显著抑制HCT116细胞增殖,抑制率与剂量和时间呈依赖关系.THHta作用HCT116细胞48、72 h的IC_(50)值小于20 μg/ml.THHta(10μg/ml)能诱导HCT116细胞G_0/G_1期比例增加及细胞凋亡,caspase-3活化及PARP蛋白剪切显著增强.结论 THHta能显著抑制HCT116细胞增殖,并可诱导HCT116细胞G_0/G_1期阻滞及细胞凋亡.
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| 关 键 词： | 昆明山海棠 生物碱 HCT116细胞 增殖 凋亡 |
Effects of total alkaloids of Tripterygium hypoglaucum Hutch on proliferation and apoptosis of human colon cancer cell line HCT116 |
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| HUANG Xiao-chun,LIU Jin-yi,ZHOU Yan-hong,AO Lin,YANG Lu-jun,CAO Jia.Effects of total alkaloids of Tripterygium hypoglaucum Hutch on proliferation and apoptosis of human colon cancer cell line HCT116[J].Acta Academiae Medicinae Militaris Tertiae,2009,31(22):2246-2248. |
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| Authors: | HUANG Xiao-chun LIU Jin-yi ZHOU Yan-hong AO Lin YANG Lu-jun CAO Jia |
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| Institution: | HUANG Xiao-chun,LIU Jin-yi,ZHOU Yan-hong,AO Lin,YANG Lu-jun,CAO Jia(Department of Military Toxicology,College of Military Preventive Medicine,Third Military Medical University,Chongqing 400038,China) |
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| Abstract: | Objective To investigate the effects of total alkaloids of tripterygium hypoglaucum hutch(THHta) on the proliferation and apoptosis of human colon cancer cell line HCT116.Methods The effects of THHta on the proliferation of HCT116 cells were assessed by MTT assay.Morphological changes of HCT116 cells were observed with invert microscopy and fluorescence microscopy.The cell cycle distribution and apoptosis of HCT116 cells were examined by flow cytometry(FCM).The expression of active caspase-3 and cleaved PAR... |
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| Keywords: | tripterygium hypoglaucum hutch alkaloids HCT116 cell proliferation apoptosis |
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