黄连水提液化学成分的分离与鉴定

目的对黄连(Coptis chinensis Franch.)的化学成分进行研究。方法采用不同柱色谱技术进行分离,通过波谱手段确定化合物结构。结果分离鉴定了22个化合物,其中9个生物碱类化合物,分别为小檗碱(berberine,1)、巴马亭(palmatine,2)、黄连碱(coptisine,3)、表小檗碱(epiberber-ine,4)、药根碱(jatrorrhizine,5)、非洲防己碱(columbamine,6)、groenlandicine(7)、木兰花碱(mag-noflorine,8)、8-氧化黄连碱(8-oxocoptisine,9);9个有机酸类化合物,分别为5-阿魏酰奎宁酸(5-O-feruloyl-D-quinic acid,10)、4-阿魏酰奎宁酸(4-O-feruloyl-D-quinic acid,11)、3-甲氧基-4-羟基苯甲酸(3-methoxy-4-hydroxybenzoic acid,12)、阿魏酸(ferulic acid,13)、香草酸-4-O-β-D-葡萄糖苷(va-nillic acid-4-O-β-D-glucopyranoside,14)、3-(3',4'-二羟基苯基)-(2R)-乳酸-4'-O-β-D-吡喃葡萄糖苷(3-(3',4'-dihydroxyphenyl)-(2R)-lactic acid-4'-O-β-D-glucopyranoside,15)、3-(4'-羟基苯基)-(2R)-乳酸(3-(4'-hydroxyphenyl)-(2R)-lactic acid,16)、3-(3',4'-二羟基苯基)-(2R)-乳酸(3-(3',4'-dihydroxyphenyl)-(2R)-lactic acid,17)、3-(3',4'-二羟基苯基)-(2R)-乳酸甲酯(3-(3',4'-di-hydroxyphenyl)-(2R)-lactic acid methyl ester,18);4个木脂素类化合物,分别为异落叶松树脂素-9-O-β-D-吡喃葡萄糖苷(isolarisiresinol-9-O-β-D-glucopyranoside,19)、(+)-松脂醇-4,4'-O-β-D-吡喃葡萄糖苷((+)-pinoresinol-4,4'-O-β-D-diglucopyranoside,20)、7S,8R,8'R-(+)-落叶松脂素-4,4'-O-β-D-吡喃葡萄糖苷(7S,8R,8'R-(+)-larisiresnol-4,4'-O-β-D-diglucopyranoside,21)、7S,8R,8'R-(+)-落叶松脂素-4-O-β-D-吡喃葡萄糖苷(7S,8R,8'R-(+)-larisiresinol-4-O-β-D-glucopyranoside,22)。结论化合物15、16、18-22为首次从黄连植物中分离得到,化合物16、18、19首次从黄连属植物中分离得到。

Chemical constituents from the decoction ofCoptis chinensis Franch.

Objective To study the chemical constituents from the decoction of Coptis chinensis Franch..Methods Compounds were isolated and purified by silica gel,sephadex LH-20,polyamide and HPLC column chromatography and their chemical structures were elucidated on the basis of physicochemical properties and spectral analyses.Results Twenty-two compounds were isolated and identified as:berberine(1),palmatine(2),coptisine(3),epiberberine(4),jatrorrhizine(5),columbamine(6),groenlandicine(7),magno-florine(8),8-oxocoptisine(9),5-O-feruloyl-D-quinic acid(10),4-O-feruloyl-D-quinic acid(11),3-methoxy-4-hydroxybenzoic acid(12),ferulic acid(13),vanillic acid-4-O-β-D-glucopyranoside(14),3-(3′,4′-dihydroxyphenyl)-(2R)-lactic acid-4′-O-β-D-glucopyranoside(15),3-(4′-hydroxyphenyl)-(2R)-lactic acid(16),3-(3′,4′-dihydroxyphenyl)-(2R)-lactic acid(17),3-(3′,4′-dihydroxyphenyl)-(2R)-lactic acidmethyl ester(18),isolarisiresinol-9-O-β-D-glucopy-ranoside(19),(+)-pinoresinol-4,4′-O-β-D-diglucopyranoside(20),7S,8R,8′R-(+)-larisiresnol-4,4′-O-β-D-diglucopyranoside(21),7S,8R,8′R-(+)-larisiresinol-4-O-β-D-glucopyranoside(22).Conclusions Compounds 16,18 and 19 are isolated from Coptis genus for the first time.Compounds 15,16,18-22 are isolated from C.Franch.for the first time.