全缘叶紫珠三萜类成分研究

为了对全缘叶紫珠的茎和叶进行化学成分研究, 采用硅胶、凝胶等柱色谱方法分离和纯化化合物, 根据理化性质和波谱方法鉴定化合物结构。从全缘叶紫珠的甲醇提取物中分离得到了15个三萜类化合物, 包括1个新化合物: 2α, 3β, 19α, 23-tetrahydroxy-olean-12-en-28-oic acid-28-O-β-D-glucopyranosyl-(1→4)-β-D-glucopyranoside (1) 和14个已知化合物: oleanolic acid (2)、3-acetyl oleanolic acid (3)、3β-O-acetyl ursolic acid (4)、2α-hydroxy-ursolic acid (5)、2α, 3β, 19α, 23-tetrahydroxy-urs-12-en-28-oic acid (6)、α-amyrin-3-O-β-D-glucopyranoside (7)、pomolic acid (8)、betulinic acid (9)、ursolic acid (10)、2α, 3β, 19α, 23-tetrahydroxy-olean-12- en-28-oic acid (11)、2α-hydroxy-oleanolic acid (12)、hederagenin (13)、2α, 19α-dihydroxy-ursolic acid (14) 和pruvuloside A (15)。所有化合物均为首次从全缘叶紫珠中分离得到, 其中化合物3、4和15为首次从紫珠属中分离得到。

Triterpenes from Callicarpa integerrima Champ

A new triterpenoid saponin and fourteen known triterpenoids were isolated from the methanol extract of the stems and leaves of Callicarpa integerrima Champ, which is used in Chinese folk medicine for stopping bleeding, expelling the wind, dissipating stagnation, and treating scrofula, by using various chromatographies, such as silica gel, Sephadex LH-20 and RP-C₁₈ column chromatography.  Their structures were identified as a new compound 2α, 3β, 19α, 23-tetrahydroxy-olean-12-en-28-oic acid-28-O-β-D-glucopyranosyl- (1→4)-β-D-glucopyranoside (1), together with fourteen known compounds: oleanolic acid (2), 3-acetyl oleanolic acid (3), 3β-O-acetyl ursolic acid (4), 2α-hydroxy-ursolic acid (5), 2α, 3β, 19α, 23-tetrahydroxy-urs-12-en-28- oic acid (6), α-amyrin-3-O-β-D-glucopyranoside (7), pomolic acid (8), betulinic acid (9), ursolic acid (10), 2α, 3β, 19α, 23-tetrahydroxy-olean-12-en-28-oic acid (arjungenin) (11), 2α-hydroxy-oleanolic acid (12), hederagenin (13), 2α, 19α-dihydroxy-ursolic acid (14) and pruvuloside A (15), by the spectroscopic techniques of NMR, HMBC, IR and MS, separately.  All these compounds were obtained from this plant for the first time, and compounds 3, 4 and 15 were isolated from genus Callicarpa L. for the first time.