含人参炔醇的胡萝卜提取物的制备工艺研究

目的 探索及优化从胡萝卜中提取人参炔醇的工艺。方法 采用高效液相色谱（HPLC）技术，以提取物中人参炔醇的含量为考察指标，对提取部位、提取方式等影响提取效率的因素分别进行单因素实验，在此基础上设计正交实验进行优化。流式细胞仪检测该工艺得到的提取物和人参炔醇体外对白血病细胞株HL-60细胞的作用。结果 确定以新鲜去汁后的胡萝卜渣为提取原料，优选工艺为1倍量石油醚超声提取3次，每次超声2 h。流式细胞仪检测显示40 μg/mL提取物和5 μmol/L人参炔醇作用12 h后细胞凋亡显著。结论 该工艺方法简便，合理可行，可显著提高胡萝卜中人参炔醇的提取率，得到的提取物体外对白血病细胞株HL-60细胞具有显著的増殖抑制和促凋亡作用。

Study on extraction technology of carrot extract containing panaxynol

Objective To investigate and optimize the technology of extraction of panaxynol from carrot. MethodsUsing the concentration of panaxynol as indicator by high performance liquid chromatography(HPLC),the factors that affected the extraction of panaxynol,including extracting parts and method were investigated respectively by single factor method.Based on the preliminary result,the orthogonal test was designed to optimize the extraction technology.Flow cytometry was employed to observe the effect of the optimized extract and panaxynol on leukemia cell line HL60 in vitro. Results The press residue was determined as the raw material of extraction.The optimum conditions were that the ratio of material to liquid(petroleum ether) was 1∶1,times of ultrasonic extraction were three,and the duration of extraction was 2 h.Significant apoptosis of HL60 cells was revealed by flow cytometry after treatment of the extract(40 μg/mL) and panaxynol(5 μmol/L) for 12 h,respectively. Conclusion The optimized extraction method is simple,convenient and reasonably practicable,with which the efficiency of extraction of panaxynol from carrot is increased significantly,and the extract has significant effects on growth inhibition and apoptosis promotion of HL60 cells in vitro.