腑安汤对小鼠术后肠麻痹的影响及机制

目的：探讨腑安汤对术后肠麻痹的治疗作用及相关机制。方法：90只小鼠随机均分为假手术组、模型组和腑安汤预处理组后常规制作术后肠麻痹模型或行假手术。应用ELISA法测定小肠组织中类胰蛋白酶、组胺含量；光镜下观察经甲苯胺蓝染色的肠系膜肥大细胞计数及脱颗粒情况；利用比色法测定小肠组织中髓过氧化物酶（MPO）活性；检测小鼠肠道内碳末移行率。结果：与假手术组比较，模型组小肠组织类胰蛋白酶、组胺含量和MPO活性均明显升高（均P<0.01），而腑安汤预处理组小肠组织类胰蛋白酶、组胺含量和MPO活性的升高受到明显抑制，与模型组比较,差异均有统计学意义（均P<0.01）；各组肥大细胞计数无统计学差异（P>0.05），但模型组细胞脱颗粒明显，而腑安汤预处理组较弱；术后模型组碳末移行率明显降低（P<0.01），腑安汤预处理组碳末移行率较模型组明显增加（P<0.01）；相关性分析显示，MPO活性与碳末移行率呈负明显相关（r=-0.885，P<0.01）。结论：腑安汤可减少术后小鼠肠道类胰蛋白酶和组胺的产生，抑制肥大细胞脱颗粒及其所介导的炎症反应，有预防和减轻术后肠麻痹的作用。

The influence of Fuan decoction on postoperative ileus of rat and its mechanism

Objective: To investigate the therapeutic effect of Fuan decoction on postoperative ileus (POI) and its related mechanisms. Methods: Ninety mice were randomized into sham operated control group, POI model group and Fuan decoction pretreatment group, and then the rats underwent the conventional procedure of POI establishment or sham operation, respectively. The content of tryptase and histamine of the small intestinal tissue was detected by ELISA method. The number of mesenteric mast cells was counted and their degranulation was observed under light microscope after toluidine blue staining. The myeloperoxidase (MPO) activity of the small intestinal tissue was determined by colorimetric method and the carbon powder moving rate was also measured. Results: The content of tryptase and histamine, and the MPO activity of the POI model group were all increased significantly compared with those of the sham operated control group (all P<0.01), but the elevations of these parameters were remarkably inhibited in the Fuan decoction pretreatment group and these had statistical differences compared with the POI model group (all P<0.01). No significant difference in the number of mast cells was found among the groups, but the degranulation of mesenteric mast cells was evident in POI model group and was inhibited in Fuan decoction pretreatment group. The carbon powder moving rate was significantly decreased in POI model group after surgery (P<0.01), which was significantly higher in Fuan decoction pretreatment group than that of the POI model group (P<0.01). Conclusion: Fuan decoction can reduce the production of intestinal tryptase and histamine after surgery and inhibit the degranulation of mast cells and their inducttion of inflammatory response, and thus it has the effect of preventing and alleviating postoperative Ileus.