ω-3不饱和脂肪酸对肝部分切除大鼠残肝的保护作用及机制

目的：探讨ω-3不饱和脂肪酸（ω-3PUFA）对肝部分切除大鼠残肝的保护作用和机制。方法：雄性SD大鼠96只随机分为:假手术组,模型组（肝部分切除+生理盐水）,低剂量ω-3PUFA治疗组（肝部分切除+1 mL/kg ω-3PUFA）,高剂量ω-3PUFA治疗组（肝部分切除+2 mL/kg ω-3PUFA）。术后1,2,3,5 d光镜下动态观察肝脏病理形态学改变,并检测术后3 d肝细胞Occludin蛋白的表达和超微结构改变。结果：病理学检查可见模型组肝细胞组织形态受损明显,而两个剂量的ω-3PUFA治疗组肝细胞受损情况明显减轻,但其两者间差异较小;术后3 d,两个剂量的ω-3PUFA治疗组Occludin蛋白表达较模型组明显升高,但其两者间差异不明显;电镜发现模型组肝细胞紧密连接严重受损,两个剂量的ω-3PUFA治疗组受损程度明显减轻,且高剂量组作用优于低剂量组。结论：肝切除术后大鼠围手术期应用ω-3PUFA可能通过增加Occludin蛋白表达和维持肝细胞间紧密连接,从而产生保护肝脏屏障的效果。

Protective effect of ω-3 polyunsaturated fatty acid on remnant liver in rat with partial hepatectomy and its mechanism

Objective: To investigate the protective effect of ω-3 polyunsaturated fatty acid (ω-3PUFA) on the remnant liver in rat with partial hepatectomy and its mechanism. Methods: Male SD rats were randomly divided into sham operated control group, model group (partial hepatectomy + normal saline), low dose ω-3PUFA treatment group (partial hepatectomy + 1 mL/kg ω-3PUFA) and high dose ω-3PUFA treatment group (partial hepatectomy + 2 mL/kg ω-3PUFA). The histopathological change of the remnant liver was dynamically assessed by light microscope at day 1, 2, 3 and 5 after surgery. In addition, the expression of occludin protein and the ultramicrostructural features of the hepatic cells were examined at day 3 after surgery. Results: The histopathological findings showed that the hepatic cells and histological structures were severely damaged, while the pathological changes of the two doses of ω-3PUFA treatment groups were of markedly lesser degree but no obvious difference was noted between themselves. The expression levels of occludin protein of the two doses of ω-3PUFA treatment groups were markedly higher than that of the model group, but were similar between themselves. The electron microscopic observations revealed that the tight junctions between hepatic cells were severely disrupted, while these structures of the two doses of ω-3PUFA treatment groups were maintained relatively intact, and the high dose group was better than that of low dose group in this respect. Conclusion: The perioperative administration of ω-3PUFA can increase the occludin expression and preserve the ultramicrostructures of hepatic cells in rat with partial liver resection, which may be responsible for its effect of hepatic barrier protection.