地龙湿法超微粉碎提取物在模拟胃肠环境中的降解研究

研究地龙湿法超微粉碎提取物在模拟胃肠环境中的降解情况。采用调整溶液pH及膜生物反应器原理两种方法终止酶解反应、Bradford法检测地龙蛋白浓度变化情况、SDS-PAGE凝胶电泳法检测地龙蛋白的降解情况、HPLC方法检测地龙小分子物质降解情况。结果表明, 地龙蛋白在人工胃液中发生完全降解; 在人工肠液中, 高分子量蛋白发生较大程度降解, 而低分子量蛋白未发现明显降解。地龙小分子物质在人工胃液中未发生明显降解; 在人工肠液中出现明显降解, 并且出现了新的小分子物质。地龙湿法超微粉碎提取物在体内发挥药效作用的物质可能是已降解后的多肽、氨基酸及可稳定存在于肠液环境的小分子物质。

Degradation of earthworm extracts prepared by wet superfine grinding in simulated gastrointestinal environment

This is to report the study of degradation of earthworm extracts prepared by wet superfine grinding in simulated gastrointestinal environment.  Enzymatic reactions were terminated by adjusting the solution pH or using membrane bioreactor principle.  Earthworm protein concentration change was detected by Bradford method, the degraded state of protein was described with SDS-PAGE technology, and the degraded state of small molecule substances was detected by HPLC.  The results showed that earthworm protein degraded completely in artificial gastric juice.  High molecular weight protein degraded greatly in artificial intestinal fluid, while low molecular weight protein was not significantly degraded.  Small molecular substances degradation did not degrade in artificial gastric juice, while they degraded obviously in artificial intestinal fluid, there is even new small molecule substance appeared.  Finally it is concluded that the substance that having therapeutic effects in vivo may be some degraded peptide, amino acid and stable small molecules existed in artificial intestinal fluid.