花青素对IgE介导的肥大细胞活化的影响

观察花青素 (anthocyanidin) 对肥大细胞活化脱颗粒的影响。通过大鼠被动皮肤过敏反应 (PCA) 实验, 采用比色测定法检测花青素在体内对肥大细胞的影响; 体外观察花青素对肥大细胞脱颗粒, 细胞内钙摄入, 肿瘤坏死因子-α (TNF-α) 及白细胞介素6 (IL-6) 释放以及p38MAPK、Akt、NF-κB磷酸化的影响。动物实验显示, 花青素 (50和100 mg·kg⁻¹) 明显抑制大鼠PCA。细胞实验显示, 花青素 (50和100 µmol·L⁻¹) 抑制肥大细胞释放组胺、TNF-α及IL-6, 并可抑制 p38MAPK、Akt和NF-κB的磷酸化。结果提示, 花青素的抗过敏作用与其抑制肥大细胞的脱颗粒, 抑制组胺、TNF-α、IL-6等炎症介质释放以及抑制细胞内钙摄入有关; 花青素抑制肥大细胞的活化可能与其抑制NF-κB、p38MAPK和Akt的活性相关。

Anthocyanidin inhibits immunoglobulin E-mediated allergic response in mast cells

This study is to investigate the anti-allergic effect of anthocyanidin and to explore its possible mechanism.  The experiments of passive cutaneous anaphylaxis reaction (PCA) and colorimetry were used to determine the effect of anthocyanidin on degranulation of mast cells in vivo.  For in vitro study, various concentrations of anthocyanidin (100, 50 and 25 µmol·L⁻¹) were added to the culture medium of mast cells cultured with 100 µg·L⁻¹ of dinitrophenyl (DNP) specific IgE overnight.  The azelastine (100 µmol·L⁻¹) was selected as the positive contro1.  The antigen (DNP-human serum albumin, DNP-HAS)-induced release of degranulation was measured by enzymatic assay, histamine was determined by EIA, and interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were measured by Western blotting, separately.  In addition, the effects of anthocyanidin on phosphorylation of NF-κB, p38MAPK and Akt were observed by Western blotting.  The results showed that treatments with anthocyanidin (100 and 50 mg·kg⁻¹) were followed by a decrease in PCA of rats.  Anthocyanidin (100 and 50 µmol·L⁻¹) obviously suppressed the degranulation from mast cells, whereas results from anthocyanidin (100 and 50 µmol·L⁻¹) group indicated significant inhibitory effect on histamine,  the calcium uptake, TNF-α, IL-6, phosphorylation of NF-κB, p38MAPK and Akt of mast cells induced by antigen.  Anthocyanidin may suppress the anaphylactic reaction by inhibiting the action of mast cells.  NF-κB, p38MAPK and Akt at least in part contribute to this event.