星点设计效应面法优化氟比洛芬纳米结构脂质载体凝胶剂的处方

目的采用星点设计法以经皮累计渗透量为指标优化氟比洛芬纳米结构脂质载体凝胶剂的处方。方法以经皮累积渗透量为评价指标,考察了凝胶基质量(x1)、氮酮用量(x2)和丙二醇用量(x3)对氟比洛芬纳米结构脂质载体凝胶剂性质的影响。用多元线性方程和二次多项式描述经皮累积渗透量和3个影响因素之间的数学关系,根据经皮累积渗透量的最佳数学模型描绘效应面,选择最佳处方,并进行预测分析。结果三个影响因素和经皮累积渗透量之间存在定量关系。优选的最佳处方:卡波姆940为0.15 g、氮酮为0.70 g,丙二醇为1.50 g。优化处方各指标的预测值和目标值接近。结论所建立的模型预测性良好,可用于预测和优化氟比洛芬纳米结构脂质载体凝胶剂处方。

Application of central composite design-response surface methodology to the formulation of flurbiprofen-loaded nanostructured lipid carriers gel

Objective To prepare and opimize the flurbiprofen-loaded nanostructured lipid carriers gel using central composite design-response surface methodology in order to obtain a formulation with optimized properties.Methods The effects of formulation variables(amount of Carbopol 940,amount of azone and amount of propylene glycol) on a number of response variables were systemically investigated.The response variables were cumulative amount of percutaneous penetration.A desirability function that combines the response variable was constructed.A second-order polynomial equation was fitted to the data,and the resulting eqution was used to predict the responses in the optimal region.Results The response variable was found to be highly dependent on the formulation variables.It was found that optimum cumulative amount of percutaneous penetration of the flurbiprofen-loaded nanostructured lipid carriers gel can be obtained at 0.15 g of Carbopol 940,0.70 g of azone,1.50 g of propylene glycol.The experimental values of the response variables highly agreed with the predict values.Conclusions Central composite design-response surface methodology is successfully used to optimize the preparation of flurbiprofen-loaded nanostructured lipid carriers gel.Overall desirability can be used to represent the most desirable variables in experimental design.