3种人参皂苷对小鼠脾淋巴细胞的生物热力学表达的影响

目的 考察3种人参皂苷对小鼠脾淋巴细胞生物热活性影响的差异，为中药活性的筛选建立新方法。方法 采用热活性检测系统，根据淋巴细胞生长代谢曲线，检测淋巴细胞的最大发热量，代谢热平均值及衰减速率常数；同时，采用MTT法对实验结果进行验证。结果 3种人参皂苷单体在不同浓度下均可使淋巴细胞的最大发热值升高，代谢热平均值上升，衰减速率常数减慢，基本趋势为人参皂苷Rg3＞人参皂苷Rg2＞人参皂苷Rg1，其中人参皂苷Rg1的作用不明显，MTT法所得的实验结果与上述结果基本一致。结论 热活性检测系统可准确检测出3种人参皂苷能够不同程度地增加脾淋巴细胞生物热活性。

Effects of 3 Genosides on Bio-thermodynamic Expression of Splenic Lymphocyte in Mice

OBJECTIVE: To establish a new method for screening active ingredients of Chinese herbs by determining different bio-thermodynamic effects of 3 genosides on splenic lymphocyte of mice. METHODS: Using a thermal bioactivity monitoring system, the maximum heat output (mHO), average metabolic heat (MH) and constant of decrease rate (DR) of lymphocyte were determined based on the growth metabolic power-time curve, and the outcomes were verified by MIT. RESULTS: The mHO and MH increased and the DR decreased after lymphocytes being exposed to the 3 genosides in different concentrations, arranged upon their potency as genoside Rg3 > genoside Rg2 > genoside Rg1 (merely insignificant effect). MTT showed the same results. CONCLUSION: Heat activity monitoring system could precisely display the different bio-thermal dynamic effects of 3 genosides on splenic lymphocyte.