小肠移植急性排斥反应中bcl-2及bax表达的意义

目的探讨bcl-2及bax的异常表达与小肠移植急性排斥反应的关系。方法选用近交系F344/N和封闭群Wistar/A大鼠建立同种异基因小肠移植模型,并随机分为同基因移植组、异基因移植组、异基因加普乐可复(FK506)治疗组和对照组,用免疫组织化学技术检测36只大鼠术后移植肠组织中bcl-2及bax在移植肠组织中的表达。结果异基因组术后第3天,bcl-2的表达显著低于对照组(P<0.05),并随着移植天数的增加,差异更加具有显著性意义(P<0.01);bax的表达与对照组比较差异无显著性意义(P>0.05);FK506治疗组bcl-2及bax表达与对照组比较,差异均无显著性意义(P>0.05)。结论移植肠组织内bcl-2表达水平可作为早期诊断急性排斥反应的一个有参考意义的指标。

Expressions of bcl-2 and bax in acute rejection of small intestinal allografts

Objective To investigate bcl 2/bax expressions and then relationship with acute rejection in small intestine transplantation. Methods Inbred F344/N and Wistar/A rats were used to establish small intestinal transplantaion model. The recipients were designed to control, isograft, allograft and allograft plus FK506 treatment groups. The control group only received laporotomy. The expressions of bcl 2 and bax was detected by immunohistochemistry. Results The expression of bcl 2 in small intestine mucous membrane epithelium during acute rejection decreased remarkedly (P< 0.05) on the third day postoperatively in allograft group, and further decreased (P< 0.01) with time. There was no significant difference in bax expression between allograft group and the control group (P >0.05). There was no significant difference in bcl 2 and bax expression between FK506 treatment group and the control group (P >0.05). Conclusion High expression of bcl 2 is an early indicator for acute rejection of small intestinal allograft.