Immobilization of Candida rugosa lipase for resolution of racimic ibuprofen |
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| Authors: | Saeid Ghofrani Abdolamir Allameh Parichehreh Yaghmaei Dariush Norouzian |
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| Affiliation: | 1.Science and Research Branch, Islamic Azad University, Tehran, Iran ;2.Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran ;3.Nano-Biotechnology Department, New Technologies Research Group, Pasteur Institute of Iran, Tehran, Iran |
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| Abstract: | AimDue to lipases’ regio-selectivity and ability to catalyze different reactions such as hydrolysis, esterification, and transesterification, the enzyme is attractive in biotransformation technology. Besides, another technology, namely enzyme immobilization, has attracted scientists/technologists’ attention to employ immobilized lipase in such a field. Thus lipase of Candida rugosa was immobilized onto silica nanoparticles through adsorption. Furthermore, the immobilized biocatalyst was characterized and used to esterify ibuprofen enantioselectively.MethodsTo characterize immobilized lipase onto silica nanoparticles scanning electron microscopy (SEM) and dynamic light scattering (DLS) were used.ResultsThe catalytic properties of both immobilized and free lipases such as optima pH and temperature were not different. According to the results, the immobilized lipase on silica nanoparticles showed 45% and 96% conversion (C) and enantioselectivity (ees), respectively. In comparison to free lipase, the immobilized enzyme came with better catalytic activity.ConclusionSilica nanoparticles as one of the most promising materials for the immobilization of lipase in enantioselective esterification of ibuprofen, were introduced in this work.Graphical abstract |
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| Keywords: | Lipase Characterization esterification Enantiomeric resolution |
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