PTEN/PI3K信号转导相关蛋白在非小细胞肺癌组织中的表达及其意义

背景与目的:PTEN/PI3K信号转导途径能调节细胞的增殖与存活,与多种肿瘤的发生发展密切相关,但在肺癌中所起的作用及其相互关系尚不十分明确。本研究旨在探讨PTEN/PI3K信号转导相关蛋白IGF-1R、PTEN和PI3K在非小细胞肺癌(non-smallcelllungcarcinoma,NSCLC)中的表达及其意义。方法:利用免疫组织化学SP法检测59例NSCLC组织、19例转移的淋巴结组织、16例癌旁增生肺组织和7例正常肺组织中IGF-1R、PTEN和PI3K蛋白的表达。IGF-1R、PTEN和PI3K蛋白阳性率的差异比较采用卡方检验和Fisher确切概率法。结果:59例NSCLC组织中IGF-1R、PTEN和PI3K蛋白阳性率分别为:72.88%、27.12%和84.75%。IGF-1R以及PI3K蛋白的阳性率与NSCLC的临床病理特征无显著相关(P>0.05)。PTEN蛋白的阳性率与NSCLC的组织学类型、细胞分化程度无关(P>0.05),但与淋巴结转移显著相关(P=0.009)。NSCLC组及转移淋巴结组中PI3K、PTEN蛋白的阳性率与正常肺组织和癌旁增生肺组织相比,差异均有显著性(P<0.05)。59例NSCLC组织中IGF和PI3K之间具有显著正相关性(P=0.001),Pearson列联系数为0.432。PTEN和PI3K之间具有显著负相关(P=0.000),Pearson列联系数为0.505。结论:IGF-1R、PI3K蛋白的过表达和PTEN蛋白的低表达与肺癌的发生及浸润转移相关。

Expression and significance of PTEN/PI3K signal transduction-related proteins in non-small cell lung cancer

BACKGROUND & OBJECTIVE: PTEN/PI3K signal transduction pathway regulates cell proliferation and survival, and is closely associated with the development and progress of various tumors. However, the relationship between the function of this pathway with lung carcinoma has not been completely elucidated. This study was to investigate the expression and clinical significance of PTEN/PI3K signal transduction-related proteins, such as insulin-like growth factor-1 receptor (IGF-1R), PTEN and phosphatidylinositol 3-kinase (PI3K), in non-small cell lung carcinoma (NSCLC). METHODS: The expression of IGF-1R, PTEN, and PI3K in 59 specimens of primary NSCLC, 19 specimens of metastatic lymph nodes, 16 specimens of para-cancerous hyperplastic lung tissues, and 7 specimens of normal lung tissues were detected by SP immunohistochemistry. The difference of positive rates of IGF-1R,PTEN and PI3K was evaluated by Chi-square test and Fisher's exact test. RESULTS: The positive rates of IGF-1R, PTEN, and PI3K in NSCLC were 72.88%, 27.12%, and 84.75%, respectively. The positive rates of IGF-1R and PI3K were not associated with the clinicopathologic features of NSCLC (P>0.05). The positive rate of PTEN was highly associated with lymph node metastasis of NSCLC (P=0.009), but was not associated with pathologic type and differentiation grade of NSCLC (P>0.05). The positive rate of PTEN was significantly lower and that of PI3K was significantly higher in NSCLC and metastatic lymph node tissues than in para-cancerous hyperplastic and normal lung tissues (P<0.05). The expression of IGF-1R in NSCLC was positively correlated to that of PI3K (r=0.432,P=0.001); while the expression of PTEN was negatively correlated to that of PI3K (r=0.505, P<0.001). CONCLUSION: Overexpression of IGF-1R and PI3K, and low expression of PTEN are closely correlated to the development, invasion and metastasis of NSCLC.