| MMP-1、MMP-13在大鼠骨关节炎软骨中的表达 |
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| 引用本文： | 马文明,董启榕,谢宗刚,李涧,周高礼. MMP-1、MMP-13在大鼠骨关节炎软骨中的表达[J]. 中国现代医药杂志, 2011, 13(3): 5-7 |
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| 作者姓名： | 马文明 董启榕 谢宗刚 李涧 周高礼 |
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| 作者单位： | 苏州大学附属第二医院骨科,215004 |
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| 摘 要： | 目的观察MMP-1和MMP-13在大鼠前交叉韧带切断加内侧半月板切除的骨关节炎模型中的表达,探讨MMP-1和MMP-13表达与骨关节炎(OA)进程的关系。方法将25只SD大鼠右膝行前交叉韧带切断加内侧半月板切除术(ACLT+MMx),对侧作为空白对照,分别于术后1周、2周、4周、6周、10周处死,每次随机处死5只,另取5只SD大鼠仅行右膝关节切开术作为假手术组,于术后10周处死,对膝关节软骨标本进行大体和组织学切片观察及评分,用ELISA法检测MMP-1、MMP-13在关节软骨中的表达。结果 ACLT+MMx组Mankin评分由1周的1.0分到4周的6.2分发展到10周的13.6分,与假手术组及空白对照组相比有显著差异(P<0.01)。ACLT+MMx组MMP-13的表达在OA早期增高,中重度期后出现明显下降趋势(P<0.01);MMP-1的表达则随着病程持续升高,与病变严重程度呈正相关性(P<0.01)。假手术组及空白对照组软骨细胞几乎不表达MMP-1及MMP-13。结论 MMPs在OA的发病机制中有重要作用,MMPs各亚型在OA进程中的表达各不相同。
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| 关 键 词： | 大鼠 骨关节炎 MMPs |
Expression of MMP-1 and MMP-13 in rat osteoarthritis cartilage |
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| Ma Wenming,Dong Qirong,Xie Zonggang,et al.. Expression of MMP-1 and MMP-13 in rat osteoarthritis cartilage[J]. Modern Medicine Journal of China, 2011, 13(3): 5-7 |
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| Authors: | Ma Wenming Dong Qirong Xie Zonggang et al. |
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| Affiliation: | Ma Wenming,Dong Qirong,Xie Zonggang,et al.The Second Affiliated Hospital of Suzhou University,Suzhou 215004 |
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| Abstract: | Objective To observe the characteristics of the expression of matrix metalloproteinase(MMPs)-1,13 in chondrocyte in the process of osteoarthritis(OA) and to explore the relation between MMPs-1,13 expression and the severity of OA.Methods OA was induced by cutting off the anterior cruciate ligament and the medial meniscus(ACLT+MMx) of their right knees,while the left knees as the control samples.The rats were executed and the samples were extracted at 1,2,4,6 and 10 weeks after the surgery with 5 rats each t... |
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| Keywords: | MMPs |
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