乌饭树叶醇提取物对四氯化碳致小鼠急性肝损伤的保护作用

目的观察乌饭树叶醇提取物对四氯化碳致小鼠急性肝损伤的保护作用。方法60只小鼠随机分为六组：正常对照组、模型组、联苯双酯组、乌饭树叶醇提取物低、中、高剂量组，用四氯化碳造成小鼠急性肝损伤模型，测定血清谷丙转氨酶（ALT）、谷草转氨酶（AST）、肝组织超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量。肝组织常规HE染色观察。结果急性肝损伤小鼠血清ALT、AST水平以及肝组织MDA含量显著升高，伴SOD活性明显下降，与正常对照组比较有显著差异。预先给予乌饭树叶醇提取物能显著改善小鼠肝损伤，降低血清ALT、AST水平以及肝组织MDA含量，并升高组织SOD活性。结论乌饭树叶醇提取物对四氯化碳致小鼠急性肝损伤有明显保护作用，机制可能与其抗氧化效应和清除自由基的作用有关。

Protective effect of the ethanol extract of vaccinium bracteatum thumb leaf on acute liver injury induced by CC14 in mice

Objective To investigate the protective effect of the ethanol extract of vacciniuin bracteatum thumb leaf on acute liver injury induced by CC14 in mice. Methods Sixty mice were allocated into 6 groups randomly, respectively as the normal control group,the model group,the positive control group,the ethanol extract low,middle and high dose group. Each group had 10 mice. Acute hepatic injury model of mice was made by using CC14. The level of ALT, AST in blood serum and SOD,MDA in hepatic tissue were observed. The hepatic tissue was HE-stained conventionally and observed. Results Acute liver injury in mice could be successfully induced by CC14 ,as demonstrated by increased serum ALT, AST levels and severe histological observations. Pretreatment with the ethanol extract of vaccinium bracteatum thumb leaf indicated its potent protective effects on acute liver injury, as demonstrated by a significant decrease in ALT, AST and MDA. Conclusion The ethanol extract of vaccinium bracteatum thumb leaf had an obviously protective effect on acute liver injury induced by CC14, which could be due to its antioxidative stress effect.