补气活血药对急性心肌梗死模型大鼠梗死心肌边缘区域SDF-1、CXCR4蛋白及mRNA表达的影响

目的:观察补气活血药对急性心肌梗死模型大鼠梗死心肌边缘区新生微血管数目以及基质细胞衍生因子-1（SDF-1）、趋化因子受体（CXCR4）蛋白及mRNA表达的影响。方法：建立大鼠急性心肌梗死动物模型，采用免疫组化SP法检测心肌组织中VIII因子相关抗原（vWF）蛋白的表达，计数微血管数目（MVC）；用Western Blot和Real-Time PCR技术检测各组大鼠梗死心肌边缘区域的SDF-1因子和其特异性受体CXCR4蛋白和基因表达情况。结果：假手术组、模型组、各给药组大鼠梗死心肌边缘区域均可见vWF因子标记染色的新生微血管，假手术组大鼠心肌中新生微血管不明显，模型组大鼠梗死心肌边缘区域可见到少量新生成的微血管，各给药组大鼠梗死心肌边缘区域可见较多新生微血管。模型组与假手术组比较差异有统计学意义（P＜0.05）；各给药组与模型组相比较差异有统计学意义（P＜0.05或P＜0.01）。与假手术组比较，模型组大鼠心肌中SDF-1、CXCR4蛋白和mRNA表达明显增多（P＜0.05或P＜0.01）；与模型组比较，各给药组SDF-1、CXCR4蛋白和mRNA表达均明显增多（P＜0.05或P＜0.01）。结论：丹参、丹参黄芪药对等药物可以促进血管的生成，推测这些药物促进血管生成的机制可能是通过促进SDF-1、CXCR4蛋白及mR原NA表达水平，从而增加SDF-1、CXCR4的含量以促进新生血管的生成。

Influence of Qi-reinforcing and Blood-activating Herbs on Protein Expression and mRNA Expression of SDF-1 and CXCR4 in Infarcted Myocardium Edge Area of Acute Myocardial Infarction Rat Model

This study was aimed to observe the influence of qi-reinforcing and blood-activating(QRBA) herbs onangiogenesis of the myocardial microvascular, SDF-1 and CXCR4 protein expression as well as mRNA expression in the infarcted myocardium edge area of acute myocardial infarction (AMI) ratmodel. The AMI rat model was established. The immunohistochemical staining method was used in the detection of vWF protein expression in the myocardial tissues. The MVC account was recorded. The SDF-1 factor and its specific receptor factor CXCR4 were detected by the western blot and realtime-PCR technique in the infarcted myocardium edge area of rats from each group. The results showed that in the new generated microvessels which were staining marked by the vWF factor can be seen in infarcted myocardium edge area of rats from the sham-operated group, model group, each medication group. The new generated microvessels in the myocardium of rats in the sham-operated group were not obvious. Small amount of new generated microvessels can be seen in rats from the model group. More new generated microvessels can be seen in rats from each medication group. The comparison between the model group and the sham-operated group showed statistical difference (P<0.05). The comparison between each medication group and the model group showed statistical difference (P<0.05 or P<0.01). Compared with the sham-operated group, SDF-1, CXCR4 and mRNA expression were obviously increased in the myocardium of rats in the model group (P<0.05 or P<0.01). Compared with the model group, SDF-1, CXCR4 protein and mRNA expression were obviously increased in the myocardium of rats from each medication group (P<0.05 or P<0.01). It was concluded that herbs such as Salvia, couplet herbs of Salvia and Astragalushad stimulation effectonangiogenesis. Mechanism of these drugs in angiogenesismay be through the promotion of SDF-1 and CXCR4 protein as well as mRNA express.