双参宁心方对大鼠心肌缺血/再灌注损伤能量代谢的影响

目的:研究双参宁心方对大鼠心肌缺血/再灌注损伤能量代谢的影响。方法:采用Wistar大鼠结扎冠状动脉左前降支40 min,再灌注2 h,建立心肌缺血/再灌注模型,大鼠随机分为6组:假手术组、模型组、曲美他嗪组(10 mg·kg^-1)、双参宁心方低、中、高剂量组(22.5,45,90 mg·kg^-1)。预防给药5 d,每日1次,末次给药后1 h,行手术。肌酸激酶同工酶MB测定试剂盒检测血清CK-MB活性;HPLC测定心肌组织ATP,ADP,AMP含量并计算TAN和EC。结果:与模型组比较,双参宁心方预防性给药可降低血清CK-MB活性,同时增加心肌ATP含量和EC水平(P<0.01 或P<0.05)。结论:双参宁心方通过保存缺血心肌ATP,维持心肌细胞能荷水平减轻缺血/再灌注损伤。

Effect of Shuangshen Ningxin formula on energy metabolism of myocardial ischemia/reperfusion rats

Objective: To investigate the effect of Shuangshen Ningxin(SSNX) formula on energy metabolism of myocardial ischemia/reperfusion rats. Method: The myocardial ischemia/reperfusion model of Wistar rats was established through the ligation of left anterior descending branch of coronary artery of for 40 min and the reperfusion for 2 h. The Wistar rats were randomly divided into six groups: the sham operation group, the model group, the Trimetazidine group (10 mg·kg^-1) and SSNX groups (22.5, 45, 90 mg·kg^-1). Preventive administration was conducted for 5 d. The operation was performed at 1 h on the day of the last administration. CK-MB assay kit was adopted to detect the activity of serum CK-MB. HPLC was used to determine ATP, ADP and AMP contents in myocardial tissues and calculate TAN and EC. Result: The preventive administration with SSNX could reduce the activity of serum CK-MB and increase ATP content and EC level in myocardial tissues (P<0.01 or P<0.05 vs. the model group). Conclusion: SSNX formula can maintain energy charge in cardiomyocytes and relieve ischemia/reperfusion injury by preserving ischemic myocardium ATP.