Box-Behnken设计-效应面法优化降糖1号胶囊的提取工艺

目的 优选降糖1号胶囊的提取工艺。方法 水回流提取，以提取时间、提取次数、料液比为考察因素，总黄酮得率为考察指标，采用Box-Behnken设计试验，效应面法分析试验结果。结果 提取时间和提取次数对实验结果具有极显著的影响，料液比对试验结果具有显著性影响，最佳提取工艺为水回流提取3次，每次59 min，料液比为23 mL·g^-1，实测总黄酮得率与预测总黄酮得率的相对偏差为-2.66%。结论 Box-Behnken设计-效应面法对该提取工艺的优化结果准确、合理、可行。

Optimization of Extractive Technology for Jiangtangyihao Capsule by Box-Behnken Design-Response Surface Methodology

OBJECTIVE To optimize the extractive technology of Jiangtangyihao capsule. METHODS Water reflux, extraction time, extraction times and solid-liquid ratio were observed as factors, total flavonoids was investigated as the indicator. Box-Behnken design-response surface methodology was used to analyze test data. RESULTS Extraction time and the number of extraction times had a very significant impact on the experimental results and the solid-liquid ratio had a significant impact on the test results. The optimum extraction conditions for Jiangtangyihao capsule were obtained as follows: extracting three times with water reflux extraction, each time was 59 min, the ratio of solid-liquid was 23 mL·g^-1. Relative deviation between the measured yield of flavonoids and predicted total yield of flavonoids was -2.66%. CONCLUSION The extraction method was accurate, rational and feasible.