| HPLC-DAD测定人参首乌胶囊中8种人参皂苷类成分 |
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| 引用本文： | 吉丽娜,冯伟红,王智民,朱晶晶,张启伟,陈两绵,李春. HPLC-DAD测定人参首乌胶囊中8种人参皂苷类成分[J]. 中国中药杂志, 2013, 38(17): 2798-2802 |
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| 作者姓名： | 吉丽娜 冯伟红 王智民 朱晶晶 张启伟 陈两绵 李春 |
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| 作者单位： | 中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700;天津中医药大学, 天津 300193;中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700;中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700;中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700;中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700;中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700;中国中医科学院 中药研究所, 北京 100700;中药质量控制技术国家工程实验室, 北京 100700 |
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| 基金项目： | 国家药品标准提高暨2015版药典科研项目;国家"十二五"支撑计划项目(2011BAI03B01) |
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| 摘 要： | 目的:建立同时测定人参首乌胶囊中8种人参皂苷类成分含量的HPLC-DAD方法。方法:采用Ultimate C18色谱柱(4.6 mm×250 mm,5 μm),流动相乙腈-水,梯度洗脱,流速1 mL·min-1,柱温30 ℃,检测波长203 nm。结果:人参皂苷Rg1,Re,Rb1,Rc,Rb2,Rb3,Rd,20(S)-Rg3分别在0.242~12.1,0.222~11.1,0.251~25.1,0.245~24.5,0.232~23.2,0.232~23.2,0.264~26.4,0.244~24.4 μg成良好的线性关系,平均回收率分别为102.7%,103.2%,101.6%,101.2%,102.0%,100.7%,101.9%,102.0%。结论:该方法简便,准确,分离效果好,无干扰,可用于人参首乌胶囊中上述8种成分的含量测定。
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| 关 键 词： | 人参首乌胶囊 人参皂苷 HPLC 含量测定 |
| 收稿时间： | 2013-03-08 |
Determination of eight ginsenosides in Renshenshouwu capsules by HPLC-DAD |
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| JI Li-n,FENG Wei-hong,WANG Zhi-min,ZHU Jing-jing,ZHANG Qi-wei,CHEN Liang-mian and LI Chun. Determination of eight ginsenosides in Renshenshouwu capsules by HPLC-DAD[J]. China Journal of Chinese Materia Medica, 2013, 38(17): 2798-2802 |
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| Authors: | JI Li-n FENG Wei-hong WANG Zhi-min ZHU Jing-jing ZHANG Qi-wei CHEN Liang-mian LI Chun |
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| Affiliation: | Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China |
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| Abstract: | |
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| Keywords: | Renshenshouwu capsules ginsenoside HPLC content determination |
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