马尾松树皮提取物对顺铂所致体外人胚肾细胞毒性的保护作用

目的: 体外探讨马尾松树皮提取物(PMBE)对顺铂所致肾毒性的保护作用,并初步探究其作用机制。方法: 体外培养人胚肾细胞HEK293,用四甲基偶氮噻唑蓝(MTT)方法分别检测PMBE、顺铂对HEK293细胞生长的影响,以及PMBE对顺铂所致HEK293细胞毒性的保护作用;并检测各组细胞内的活性氧(ROS)、丙二醛(MDA)和谷胱甘肽(GSH)含量,以及过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和硫氧还蛋白酶(TrxR)的活性。结果: PMBE低浓度下促进HEK293细胞生长,较高浓度下对细胞产生轻微毒性;顺铂抑制HEK293细胞生长,使细胞内ROS,MDA含量升高,GSH含量降低,同时降低SOD,CAT和TrxR酶活性;加入PMBE预保护后,在一定范围内降低顺铂对HEK293细胞的损伤,降低ROS,MDA含量,升高GSH含量,以及提高SOD,CAT和TrxR酶活性。结论: 一定浓度的PMBE对顺铂所致人胚肾细胞的毒性有保护作用,其机制可能与PMBE的抗氧化性密切相关。

Protective effect of Pinus massoniana bark extracts against cisplatin- induced nephrotoxicity in human embryonic kidney cells

Objective: To investigate the in vitro protective effect of Pinus massoniana bark extracts (PMBE) against cisplatin-induced nephrotoxicity in human embryonic kidney cells (HEK293), and preliminarily study its mechanism. Method: Human embryonic kidney cells (HEK293) were cultured in vitro. The MTT assay was adopted to test the effect of PMBE and cisplatin on growth of HEK293 cells, and the protective effect of PMBE on cisplatin-induced nephrotoxicity of HEK293, and then detect the intracellular reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH) content, catalase (CAT), superoxide dismutase (SOD) and activity of thioredoxin reductase (TrxR). Result: PMBE could promote growth of HEK293 cells at low concentrations, but generate slight nephrotoxicity at high concentration. Cisplatin could inhibit growth of HEK293 cells, increase ROS and MDA content, while reducing SOD, CAT and TrxR. The pre-protective PMBE was added to reduce cisplatin's injury to HEK293 cells, ROS, MDA and GSH content, SOD, CAT and TrxR within certain range. Conclusion: PMBE at specific concentration has the protective effect in cisplatin-induced nephrotoxicity in HEK293 cells. Its mechanism may be related to PMBE's antioxidant activity.