反义寡核苷酸联合磁性载药微球靶向治疗耐药裸鼠人胰腺癌

目的 观察辐射及多药耐药基因逆转剂反义寡核苷酸( mdr1ASON)联合5-氟尿嘧啶磁性白蛋白微球(5-Fu-MAMS)靶向治疗耐药裸鼠人胰腺癌模型的效果.方法 构建人胰腺癌耐药细胞株(SW1990/Fu),建立裸鼠人胰腺癌耐药模型,选择适当的磁场,施加于肿瘤表面,瘤内注射mdr1ASON及5-Fu-MAMS,观察肿瘤生长,检测其对耐药裸鼠人胰腺癌的治疗效果.结果 在外加磁场的磁导向作用下,5-Fu-MAMS能定位于肿瘤组织,联合mdr1ASON能显著抑制肿瘤生长,肿瘤体积抑制率达到85.00％,肿瘤重量抑制率达到87.74％,与其他组比较差异有统计学意义(P＜0.01).结论　辐射促转染的mdr1ASON联合磁性载药微球对肿瘤细胞具有较好的耐药逆转作用.

Reversal of multidrug resistance antisense oligodeoxynucletedes and 5-fluorouracil magnetic albumin miscrospheres on human pancreatic carcinoma model in nude mice

Objective To investigate the reversal the multidrug resisitance ( MDR) by MDR gene mdrl antisense oligodeoxynucletdes (ASON) and 5-fluorouracil magnetic albumin miscrospheres (5-FuMAMS) magnetic targeted therapy the human pancreatic cancer in nude mouse model.Methods To construct SW1990/Fu and nude mice experimental model,to investigate the targeted therapy effects of mdr1ASON and 5-Fu-MAMS.Results 5-Fu-MAMS magnetic response was good,its could be well localized in the tumor tissue and markedly inhibit the growth of tumor.The inhibitory rate of tumor size was 85.00％,and that of tumor weight 87.74％.which can effectively degrade proliferating index and accelerate cell death of tumor cell.There were significant differences as compared with other groups( P ＜0.01 ).Conclusion Mdr1 ASON and 5-Fu-MAMS had outstanding reverse effect drug fast on human pancreatic carcinoma model in nude mice.