大鼠局灶性脑缺血再灌注后脑组织中脑钠肽表达的变化

目的 探讨局灶性脑缺血再灌注大鼠脑组织中脑钠肽(BNP)水平的变化. 方法 按照随机数字表法将成熟雄性Wistar大鼠100只分为假手术组、脑缺血再灌注组(再灌注组),再灌注组采用Longa法造模.2组按造模/手术后不同观察时间分为0h、3h、6h、9h、12h、24 h、48 h、72 h、1周、2周10个亚组,每亚组5只.应用HE染色、免疫组化法观察各组大鼠脑组织中BNP表达情况及病理变化. 结果 HE染色结果显示再灌注组大鼠脑组织出现神经元变性、坏死,细胞周围水肿；免疫组化染色结果显示各时间点再灌注组大鼠BNP含量均明显高于假手术组,差异有统计学意义(P＜0.05),其中造模48 h后大鼠水肿及BNP表达达到高峰. 结论 局灶性脑缺血再灌注大鼠脑组织中BNP表达增高,考虑脑水肿为重要原因.

Expression changes of brain natriuretic peptide in brain tissues of rats after focal cerebral ischemia-reperfusion

Objective To investigate the changes of brain natriuretic peptide (BNP) level in the　brain tissues of rats after focal ischemia-reperfusion. Methods Mature male Wistar rats were　randomly divided into sham-operated and ischemia-reperfusion groups. Focal cerebral　ischemia-reperfusion rat models in the ischemia-reperfusion group were established using Longa's　suture-occluded method.And the 2 groups were sub-grouped into 0,3,6,9,12,24,48 and 72 h,and 1and 2 w observation groups according to different times after ischemia-reperfusion. The pathological　changes in the brain tissues were observed by HE staining and immunohistochemistry. Results HE　staining indicated that neuron degeneration,necrosis and tissue edema could be seen in the brain tissues　of rats after ischemic-reperfusion. The BNP levels in the ischemic-reperfusion sub-groups were　significantly higher than those in the sham-operative sub-groups at corresponding time points (P＜0.05);and the peak level of edema and BNP expression appeared at 48 h after reperfusion. Conclusion The　expression of BNP in the brain tissues of rats after focal cerebral ischemia-reperfusion increases,which　might be related to the cerebral edema.