微柱凝胶卡式法Coombs试验在自身免疫性溶血性贫血诊断中的应用

目的 采用微柱凝胶卡式检测方法进行直接Coombs试验,探讨其在自身免疫性溶血性贫血( AIHA)诊断中的临床意义.方法 对临床怀疑为AIHA的128例患者标本同时用试管法和微柱凝胶卡式法进行直接Coombs试验,对两种检测方法进行比较.检测患者同期血红蛋白水平、网织红细胞比例、胆红素及游离血红蛋白水平,比较不同型别患者间的差异.结果 应用微柱凝胶卡式法进行直接Coombs试验阳性检出率为88.4％,试管法阳性检出率为37.7％,患者红细胞包被不同免疫球蛋白时溶血程度不同.结论 微柱凝胶卡式法较试管法敏感,操作简单,易标准化,结果判读客观,可长期保存.对AIHA的诊断具有临床意义.

Application of micro-column gel cards assay for direct Coombs test in diagnosis of autoimmune hemolyric anemia

Objective To evaluate the clinic value of micro-column gel cards direct Coombs test (gel test) in dignosis of autoimmune hemolytic anemia (AIHA).Methods Specimens of 128 suspected AIHA patients were performed direct Coombs test by conventional tube or gel cards.The results of the two tests were compared.The hemoglobin concentrations,proportions of reticulocyte,serum levels of bilirubin and free hemoglobin were detected simultaneously and compared in subgroups.Results The positive detection rate of direct Coombs test performed by gel test or tube were 88.4％ and 37.7％,respectively.Conclusion Compared with the tube tests,gel test assay is more sensitive,easy to perform and standardized in diagnosis of AIHA,and the gel card can be stored for a long time.The gel test is valuble for the diagnosis of AIHA.