不同产地栀子皮和栀子仁中有效成分的含量比较

目的对10个不同产地的栀子,不同药用部位(栀子果实、栀子皮、栀子仁)中有效成分栀子苷、西红花苷-Ⅰ、西红花苷-Ⅱ的含量进行比较,为栀子皮、栀子仁分开使用寻找科学依据。方法采用变换波长RP-HPLC法同时测定栀子苷和西红花苷-Ⅰ、西红花苷-Ⅱ的含量。Thermo ODS色谱柱C1(8250 mm×4.6 mm,5μm),甲醇-0.1%磷酸水梯度洗脱,流速1.0 mL.min-1,检测波长分别为栀子苷238 nm、西红花苷-Ⅰ和西红花苷-Ⅱ440 nm。结果被测定成分色谱峰与其他色谱峰可以达到完全分离。栀子苷、西红花苷-Ⅰ、西红花苷-Ⅱ的进样量分别在0.420～5.252,0.199～2.495,0.023～0.288μg范围内与各自峰面积呈良好线性关系,r分别为0.9993,0.9998,0.9997;三者平均回收率分别为98.23%,97.88%,97.77%;RSD为0.52%,2.02%,1.47%。结论该方法简便、准确、重复性好,可同时测定多个产地栀子不同药用部位栀子有效成分的含量。为栀子皮、栀子仁分别使用提供了科学依据。

Study on Effective Constituent Contents in Different Positions of Gardenia jasminoides from Different Producing Places

Objective To compare the contents of effective constituents of gardenoside,crocin-Ⅰ and crocin-Ⅱ in the fruits,seeds and pericarp of Gardenia jasminoides from different producing places.Methods Wavelength conversion RP-HPLC method was used.A Thermo ODS C18(250 mm×4.6 mm,5μm)column was used with the mobile phase consisting of acetonitrile-0.1%phosphoric acid(gradient elution) at the flow rate of 1.0 mL·min-1.The UV detection wavelength was set at 238 nm for gardenoside and 440 nm for crocin-Ⅰ and crocin-Ⅱ.Results The measured peaks were completely separated from the peaks of other components.The linear ranges of gardenoside,crocin-I and crocin-II were 0.420 ～ 5.252 μg(r=0.9993),0.199 ～ 2.495 μg(r=0.9998) and 0.023 ～ 0.288 μg(r=0.9997),respectively.The average recovery rates of gardenoside,crocin-Ⅰand crocin-Ⅱwere 98.23%(RSD=0.52%,n=6),97.88%(RSD=2.02%,n=6) and 97.77%(RSD=1.47%,n=6),respectively.Conclusion The method is sample,accurate and reproducible for the effective constituent contents in different positions of Gardenia jasminoides from different producing places.