| pcDNA3．1（＋）／hTERT-tk真核表达质粒的构建及其在HepG-2细胞中的表达 |
| |
| 引用本文： | 刘京龙,邓志华,杨长青,刘燕. pcDNA3．1（＋）／hTERT-tk真核表达质粒的构建及其在HepG-2细胞中的表达[J]. 山西中医学院学报, 2009, 10(1): 56-57,60 |
| |
| 作者姓名： | 刘京龙 邓志华 杨长青 刘燕 |
| |
| 作者单位： | 山西医科大学第二医院,山西太原,030001 |
| |
| 摘 要： | 目的：研究人端粒酶逆转录酶（hTERT）基因核心启动子调控的人单纯疱疹病毒胸苷激酶基因／更昔洛韦（HSV-tk／GCV）系统对肝癌HepG-2细胞的体外杀伤作用。方法：构建hTERT基因启动子调控的tk基因真核表达载体pcDNA3．1（＋）／hTERT-tk，用脂质体法分别转染肝癌细胞（HepG-2）和正常肝细胞（L-02）后给予更昔洛韦（GCV），用TUNEL法观察自杀基因对肝癌细胞生长的影响。结果：HETRT启动子调控下的HSV-tk／GCV系统对肝癌HepG-2细胞有明显的杀伤作用，而对正常肝细胞则作用不明显。结论：hTERT-tk／GCV基因体系能够靶向杀伤肝癌细胞，有靶向治疗肝癌的潜力。
|
| 关 键 词： | 靶向基因治疗 h-TERT启动子 自杀基因 |
Construction of pcDNA3.1(+)/hTERT-tk Eukaryotic Expression Plasmid and Its Expression in HepG-2 Cells |
| |
| Liu Jinglong,Deng Zhihua,Yang Changqing,Liu Yah. Construction of pcDNA3.1(+)/hTERT-tk Eukaryotic Expression Plasmid and Its Expression in HepG-2 Cells[J]. Journal of Shanxi College of Traditional Chinese Medicine, 2009, 10(1): 56-57,60 |
| |
| Authors: | Liu Jinglong Deng Zhihua Yang Changqing Liu Yah |
| |
| Affiliation: | Second Affiliated Hospital of Shanxi Medical University;Taiyuan Shanxi 030001 |
| |
| Abstract: | Objective:To observe the killing effect of HSV-tk/GCV system driven by human telomerase reverse transcrip-tase(hTERT)core promoter against human hepatocellular carcinoma cell line HepG-2 in vitro. Methods:pcDNA3.1(+)/ hTERT-tk eukaryotic expression plasmid containing HSV-tk gene under the control of hTERT core promoter was con-structed and was transfected into hepatoma cells (HepG-2)and normal hepatic cells (L-02)by liposome method. Then transfected cells were selected with G418,at last GCV was added and ce... |
| |
| Keywords: | target gene therapy h-TERT promoter suicide gene |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
