零下非结冰UW液保存人工肝用C3A细胞

目的探索零下非结冰（-0.8℃）保存C3A肝细胞的效果以及同细胞凋亡的关系。方法UW液（University of Wisconsin Solution）保存的C3A细胞分为以下2组：-0.8℃组,4℃组。低温保存24h、48h及72h后分别测定细胞存活率及凋亡率、AST释放、尿素合成功能及白蛋白分泌功能。结果零下非结冰（-0.8℃）72hC3A细胞存活率（86.49±2.80）%高于4℃（77.83±3.40）%,P〈0.001;细胞凋亡率（1.26±0.84）%低于4℃（9.16±1.99%,P〈0.001;AST释放（5.30±0.42）U/L低于4℃（8.18±1.05）U/L;细胞尿素合成功能（0.68±0.06）mmol/L优于4℃（0.40±0.07）mmol/L,P〈0.001;白蛋白分泌功能（2.06±0.22）mg/ml优于4℃（1.68±0.18）mg/ml（P=0.002）。结论0.8℃的UW液可明显提高低温保存C3A细胞存活率,有效地保护细胞尿素合成和白蛋白分泌功能。

Storage of artificial liver C3A cells in nonfreezing University of Wisconsin solution at a temperature below zero

Objective To study the storage of C3A liver cells in a nonfreezing solution at a temperature below zero（-0.℃8） and its relation with cell apoptosis.Methods C3A liver cells suspended in University of Wisconsin（UW） solution were divided into nonfreezing group（-0.8℃） and control group（4℃）.After 24,48 and 72h,viability and apoptosis of liver cells,release of AST and ability of liver cells to synthesize urea and secrete albumin were detected.Results The viability of liver cells was 86.49%±2.80% and 77.83%±3.40% in the nonfreezing and control groups,respectively（P0.001）.The apoptosis of liver cells was 1.26%±0.84% and 9.16%±1.99% in the nonfreezing and control groups,respectively（P0.001）.The release of AST was significantly suppressed（P0.001）.The ability of liver cells to synthesize urea and secrete albumin was better in the nonfreezing group than in the control group（P0.001）.Conclusion Storage of C3A hepatocytes in nonfreezing UW solution at a temperature below zero（-0.8℃） can increase the viability of liver cells and their ability to synthesize urea and secrete albumin.