| 液质联用法测定金樱子中熊果酸和齐墩果酸的含量 |
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| 引用本文： | 严志宏,万彦婷,卢珂,管咏梅,熊瀚海,朱根华.液质联用法测定金樱子中熊果酸和齐墩果酸的含量[J].江西中医学院学报,2010,22(6):43-45. |
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| 作者姓名： | 严志宏 万彦婷 卢珂 管咏梅 熊瀚海 朱根华 |
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| 作者单位： | [1]江西中医学院现代中药制剂教育部重点实验室,南昌330004 [2]武警江西总队医院,南昌330001 [3]浙江省肿瘤医院放疗科,杭州310022 |
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| 基金项目： | "中药新型给药系统技术平台"十一五重大新药创制基金，江西省科技支撑计划基金 |
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| 摘 要： | 目的：建立金樱子中熊果酸和齐墩果酸含量测定方法。方法：采用液质联用（LC-MS/MS）法,色谱柱Diamonsil C18（2）（250 mm×2.1 mm,3μm）,流动相甲醇-甲酸水（87∶13）,流速0.25 ml.min-1。柱温30℃,质谱采用大气压电喷雾离子源,选择性离子流模式（负离子）。结果：熊果酸和齐墩果酸的平均样回收率分别为为98.1%和97.6%,RSD分别为2.7%和2.9%。结论：该方法快速简便,灵敏度高,重现性好。可用于金樱子的质量控制。
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| 关 键 词： | 液质联用 金樱子 熊果酸 齐墩果酸 |
Content Determination of Ursolic Acid and Oleanolic Acid in Rosae Laevigata michx by HPLC-MS/MS |
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| YAN Zhi-hong,WAN Yan-ting,LU Ke,GUAN Yong-mei,XIONG Han-hai,ZHU Gen-hua.Content Determination of Ursolic Acid and Oleanolic Acid in Rosae Laevigata michx by HPLC-MS/MS[J].Journal of Jiangxi College of Traditional Chinese Medicine,2010,22(6):43-45. |
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| Authors: | YAN Zhi-hong WAN Yan-ting LU Ke GUAN Yong-mei XIONG Han-hai ZHU Gen-hua |
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| Institution: | 1.Jiangxi University of TCM,Nanchang 330004;2.Chinese People＇s Armed Police Forees Hospital,Nanchang 330001;3.Zhejiang Cancer Hospital,Hangzhou 310022 |
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| Abstract: | Objective：To establish an HPLC-MS/MS method for determination of the content of Ursolic Acid and Oleanolic Acid in Rosae Laevigata michx.Methods：Chromatograthy was performed on a 3μm Diamonsil C18（2） column with the column temperature at 30℃.and the sample size at 10μl.The mobile phase composition was methanol～water（0.2%formic acid）（87∶13）.The flow rate was 0.25 ml ·min.Results：The Ursolic Acid had a good linearity relationship（r=0.999 1,n =6） between 5.08～1016 ng/ml;The Oleanolic Acid had a good linearity relationship（r=0.999 2,n=6） between 6.46～1028 ng/ml.The averagerecovery of Ursolic Acid and Oleanolic Acid in Rosae Laevigata michx was 98.1%（RSD：2.7%） and 97.6%（RSD：2.9%）.CONCLUSIONS：This method is simple,accurate and reproducible. |
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| Keywords: | HPLC-MS/MS Rosae Laevigata michx：Ursolic Acid Oleanolic Acid Content determination |
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