兔股骨头坏死钻孔减压模型的相关研究

目的评估兔股骨头钻孔减压后细胞注入的可行性。方法模拟临床手术,对20只股骨头坏死的新西兰兔于透视下行经皮股骨头钻孔减压及0.9%氯化钠注射液注入,并进行Micro-CT、组织学及免疫组化检查;对4只新西兰免进行经皮股骨头钻孔减压及印度墨水注入,观察墨水弥散情况。结果以2mm克氏针及骨髓穿刺针可有效完成钻孔减压及0.9%氯化钠注射液注入,印度墨水注入后分布较为均匀。结论模拟临床钻孔减压手术向兔股骨头内注入细胞悬液是可行的。

Trephination decompression model of rabbit femoral head osteonecrosis

Objectives To evaluate the feasibility of infusing cell suspension into rabbit femoral head after trephination decompression.Methods Trephination of femoral head was performed in 20 and 4 New Zealand rabbits with femoral head osteonecrosis,0.9% sodium chloride solution and Indian ink were injected respectively into the femur under X ray guidance for its decompression.Micro-CT,histology and immunochemistry were used to show the distribution of sodium chloride solution and Indian ink.Results Trephination decompression of femoral head could be achieved using 2mm Kirschner wire and bone marrow aspiration needle.The sodium chloride solution and Indian ink were uniformly distributed.Conclusion Femoral head osteonecrosis can be treated with trephination decompression of femoral head and infusion of cell suspension into femoral head of rabbits.