泌尿生殖道感染支原体检测及耐药性分析

目的了解泌尿生殖道感染患者解脲脲原体(Uu)和人型支原体(Mh)的分离及耐药情况,以指导临床合理用药。方法应用法国生物梅里埃公司生产的Mycoplasma IST2试剂盒,对791例泌尿生殖道标本进行支原体培养及药物敏感试验,统计解脲脲原体和人型支原体的检出率,分析其耐药性。结果 791例标本中,支原体培养阳性492例,总阳性率为62.2%。492例阳性标本中,Uu阳性占阳性标本的70.33%,Uu与Mh均阳性占28.86%,仅Mh阳性占0.81%;其中男性支原体培养阳性率为37.50%,女性支原体培养阳性率为66.97%,女性明显高于男性(P0.05)。药敏试验结果显示,支原体对原始霉素、强力霉素、四环素、交沙霉素较敏感,对环丙沙星、氧氟沙星、罗红霉素耐药程度相当高。结论生殖道支原体感染以Uu为主,青壮年的感染率较高;支原体对多种抗生素均具有耐药性,特别是Uu与Mh混合感染时。临床治疗支原体感染应首选原始霉素、强力霉素、四环素、交沙霉素等药物。

Detection of mycoplasma in urogenital tract infection and antibiotic resistance analysis

Objective To study ureaplasma urealyticum（Uu）and mycoplasma hominis（Mh）infection in the urogenital tract infections and their drug-resistance for guiding rational clinical drug use.Methods Mycoplasma IST2 kit was used to detect Uu and Mh and their drug-resistance in the urogenital samples of 791 cases.Results Among 791 suspected cases,492 cases were positive,the positive rate was 62.2%.Among them,70.33% was infected by Uu,28.86% by Uu and 0.81% by Mh.The prevalence of mycoplasma in male was 37.50% and 66.97% in female,the positive rate of the female was much higher than that of the male（P〈0.05）.The results of drug-resistance test showed that mycoplama was much sensitive to pristinmycin,doxycycline,tetracycline,josamycin,and much resistant to ciprofloxacin,ofloxacin,erythromycin.Conclusion The urogenital mycoplasma infection results mainly from Uu,especially in young and mid-aged people.Uu or Mh infection,especially mixed infection with Uu and Mh has significantly greater resistance to a wider variety of drugs.Pristinmycin,doxycycline,tetracycline,and josamycin are the primary choices for urogenital mycoplasma infection.