油酸在小鼠体内代谢生成9,10-二羟基硬脂酸的实验观察

目的探索油酸（OA）在小鼠体内能否代谢生成9,10-二羟基硬脂酸（DHSA）。方法将4周龄雄性昆明小鼠30只适应饲养1周后按体质量随机分为3组,分别以普通饲料、含13%橄榄油饲料和含13%大豆油饲料喂养,10周后空腹12h取血测定血清、红细胞膜中的油酸和DHSA含量。结果橄榄油组血清、红细胞膜中油酸和DHSA含量显著增加,高于大豆油组和普通饲料组（P〈0.05）,但大豆油组和普通饲料组油酸和DHSA含量相近。结论油酸可在小鼠体内转化生成DHSA。

Metabolic 9,10-dihydroxystearic acid produced by oleic acid in mice

Objective To study the metabolic 9,10-dihydroxystearic acid（DHSA） produced by oleic acid（OA） in mice.Methods Thirty Kunming male mice at the age of 4 weeks were randomly divided into three groups.Ten weeks after the mice were fed with ordinary food,food containing 13% olive oil and food containing 13% bean oil,respectively,12-hour fasting blood was collected to measure the levels of OA and DHSA in serum and erythrocyte membranes.Results The levels of OA and DHSA in serum and erythrocytes membranes were significantly higher in the group fed with olive oil than in the other two groups（P0.05）.No significant difference was found in the levels of OA and DHSA between the group fed with bean oil and the group fed with ordinary food.Conclusion DHSA can be produced by the conversion of OA in mice.