392例甲型H1N1流感患者血清补体C3、C4和CH50的变化

目的探讨甲型H1N1流感患者病程初期血清补体C3、C4和CH50的变化及临床意义。方法采用免疫透射比浊法及脂质体免疫测定法分别检测369例轻症甲型H1N1流感（轻症组）和23例重症甲型H1N1流感（重症组）患者病程5d内血清中补体C3、C4水平及总补体活性（CH50）。结果轻症组C3值为（1.10±0.18）g/L,重症组为（1.10±0.19）g/L;轻症组C4值为（0.29±0.08）g/L,重症组为（0.29±0.08）g/L;76%的患者病程早期CH50出现轻微上升,15%出现明显下降;轻症组CH50值为（48.84±19.38）U/ml,重症组为（57.48±13.25）U/ml（P=0.036）。结论甲型H1N1流感患者病程早期血清C3和C4水平均在正常较高水平,CH50多轻微上升,部分明显下降。重症甲型H1N1流感CH50水平较轻症升高,有一定预警意义。

Changes of serum complement components C3,C4 and CH50 in 392 patients with H1N1 influenza A

Objective To study the changes of serum complement components C3, C4 in patients with H1N1 influenza A. Methods Serum C3, C4 levels and serum CH50 level were measured by turbindimetric immunoassay（TIA） and liposome immunoassay, respectively, in 369 patients with mild H1N1 influenza A（group A） and 23 patients with severe H1N1 influenza A（group B） during the initial 5 days after admission. Results The mean level of C3 was 1.10±0.18g/L and 1.10±0.19g/L, respectively, while the mean level of C4 was 0.29±0.08g/L and 0.29±0.08g/L, respectively, in groups A and B. The CH50 level was either slightly elevated（76%） or significantly decreased（15%）. The mean level of CH50 was 48.84±19.38U/ml and 57.48±13.25U/ml, respectively in groups A and B（P=0.036）. Conclusion Serum C3 and C4 levels are higher than normal and CH50 level is either slightly elevated or significantly decreased in patients with H1N1 influenza A at its early stage. The CH50 level is higher in patients with severe H1N1 influenza A than in those with mild H1N1 influenza A, which is of certain significance for the early warning of severe H1N1 influenza A.