A uniaxial bioMEMS device for quantitative force-displacement measurements |
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Authors: | David B Serrell Tammy L Oreskovic Andrew J Slifka Roop L Mahajan Dudley S Finch |
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Institution: | (1) National Institute of Standards and Technology, Boulder, CO, USA;(2) University of Colorado, Boulder, CO, USA;(3) University of Virginia Technology, Blacksburg, VA, USA |
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Abstract: | There is a need for experimental techniques that allow the simultaneous imaging of cellular cystoskeletal components with
quantitative force measurements on single cells. A bioMEMS device has been developed for the application of strain to a single
cell while simultaneously quantifying its force response. The prototype device presented here allows the mechanical study
of a single, adherent cell in vitro. The device works in a fashion similar to a displacement-controlled uniaxial tensile machine. The device is calibrated using
an AFM cantilever and shows excellent agreement with the calculated spring constant. The device is demonstrated on a single
fibroblast. The force response of the cell is seen to be linear until the onset of de-adhesion with the de-adhesion from the
cell platform occurring at a force of approximately 1500 nN. |
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Keywords: | Cell mechanics BioMEMS Fibroblast Microfabrication De-adhesion |
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