| 免疫金银染色法对日本血吸虫31／32KD分子抗原的定位研究 |
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| 引用本文： | 汪世平,曾宪芳,易新元. 免疫金银染色法对日本血吸虫31／32KD分子抗原的定位研究[J]. 中南大学学报(医学版), 1994, 0(1) |
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| 作者姓名： | 汪世平 曾宪芳 易新元 |
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| 作者单位： | Departwent of Parasitology，Hunan Medical University |
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| 基金项目： | TDR／WHO资助课题 |
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| 摘 要： | 应用免疫金银染色法（ＩＧＳＳ）和免疫酶染色法（ＩＥＳ）对日本血吸虫３ｌ／３２ＫＤ分子抗原进行了比较定位研究。结果证实了日本血吸虫３１／３２ＫＤ抗原是一种肠相关抗原，并进一步验证了常规制备的单特异抗血清用于抗原分析和来源定位的可行性。
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| 关 键 词： | 日本血吸虫；血吸虫病；抗原，免疫反应；染剂和染色法；免疫酶技术；兔 |
SCHISTOSOMA JAPONICUM:LOCALIZATION OF THE 31/32KD ANTIGENIC MOLECULES IN ADULT WORM SECTION BY IMMUNOGOLD-SILVER STAINING |
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| Wang Shiping, Zeng Xianfang, Yi Xinyuan. SCHISTOSOMA JAPONICUM:LOCALIZATION OF THE 31/32KD ANTIGENIC MOLECULES IN ADULT WORM SECTION BY IMMUNOGOLD-SILVER STAINING[J]. Journal of Central South University. Medical sciences, 1994, 0(1) |
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| Authors: | Wang Shiping Zeng Xianfang Yi Xinyuan |
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| Abstract: | ections of the female and the male Schistosoma japonicum were incubated withmonospecific antiserum for the 3l/32KD antigen. Immunogold-silver staining(IGSS)and Im-muno-enzyme staining(IES)showed that the antigens were present only on epithelial cells ofthe gut,but not on the tegumental surface or on the parenchyma of the adult worms. The re-sult indicated that the 31/32 KD antigen was a gutrelated antigen. In addition,the resultsdemonstrated that(1)the method of producing monospecific antiserum provided a simple wayto identify and characterize useful antigens and(2)the use of the male adult worm sectionwas better than that of the female section in IGSS,The opposite situation was observed inIES. |
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| Keywords: | Schistosomna japonicum chistosomiasis antigen immune response stains and staining immunoenzyme technic rabbits |
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