痛风克方中寒热药对不同配比对大鼠痛风模型的影响

目的:研究痛风克方中虎杖桂枝寒热药对的不同配比对大鼠痛风病理模型的影响.方法:选用SD大鼠,分为正常组、模型组、秋水仙碱0.02 g·kg-1组、虎杖-桂枝=3:1 7.5g·kg-1组(A组),虎杖-桂枝6:1 9.3 g·kg-1组(B组),(虎杖+土茯苓)-桂枝6:1 9.3 g·kg-1组(C组),各组均连续ig给药7d,给药第5天运用微晶型尿酸钠(MSU)法造模.注射MSU混悬液造模后1,2,6,8,12,24h期间,量度各组大鼠足踝关节下0.5 mm处直径和圆周长,计算出足肿胀率；造模2d后于眼眶后静脉取血检测大鼠血清尿酸及PGE2水平；摘取右后足踝关节于光镜下观测大鼠足踝关节滑膜组织变化及病理形态学改变.结果:各组方对足肿胀均能起到不同程度的抑制效果,C组方在致炎后24h足肿胀率为18.2％,仍显示出明显的抑制作用(P＜0.05)；B、C组方均能降低大鼠血清尿酸水平,分别为77.8 μmol·L-1和60.1 μmol· L-1 (P ＜0.05),降低PGE2水平,分别为9-0 pg·L-1和9.1 pg·L-1(P＜0.05),对改善足踝关节滑膜组织炎症均有较好效果,炎症变化分值可降至0.7;病理观察显示,C组滑膜及周围软组织内见少量炎细胞浸润,部分小血管扩张充血；B、C组方在各项检测指标上均优于其它组别.结论:痛风克方中寒热药对以6:1进行配伍是较好的配伍比例,对大鼠痛风病理模型的足肿胀度、血清尿酸及PGE2水平和足踝关节滑膜组织病理改变都有较好的改善作用.

Study of Different Ratio of Cold-heat Compatibility of Tongfengke in Rat Gout Model

Objective: To study the influence of the different ratio of cold-heat compatibility of Polyonum Cuspidatum(PC) and Ramulus Cinnamomi(RC) of Tongfengke in rat gout model.Method: SD rats were divided into normal group,the model group,PC-RC 3∶ 1(A group),PC-RC 6∶ 1(B group) and(PC+SX)-RC 6∶ 1(C group) and positive medicine Colchicine group,the treatment groups were given corresponding drugs for 7 days.At 5th day using micro crystal uric acid sodium(MSU) method was used to reduce model,.After 2,6,8,12,24 h,of injecting MSU suspension the diameter and circumference of ankle joint were measured,and the foot swelling rate was calculated;serum uric acid and PGE2 level were determined two days after modeling The right rear ankle joint was collected to observe the ankle joint synovial organization and the pathological morphology change by light microscopy.Result: The foot swelling was inhibited,C formula at 24 h showed amarked inhibition(18.2%,P<0.05);B,C formula could reduce serum uric acid levels(77.8 μmol·L-1 and 60.1 μmol·L-1,P<0.05)and PGE2 level(9.0 pg·L-1 and 9.1 pg·L-1,P<0.05) Inflammation change score could drop to 0.7;C formula showed a few inflammatory cell in synovial and soft tissue,the dilated small blood vessels were found;The actions of B and C grous were superior to other divisions.Conclusion: Cold-heat compatibility of Tongfengke with 6∶1 ratio is a good compatibility proportion;and can improve pathological changes.