| HPLC测定竹叶柴胡中黄酮类成分的含量 |
| |
| 引用本文： | 林海霞,王书林,王砚,孙翠萍. HPLC测定竹叶柴胡中黄酮类成分的含量[J]. 中国实验方剂学杂志, 2012, 18(15): 76-79 |
| |
| 作者姓名： | 林海霞 王书林 王砚 孙翠萍 |
| |
| 作者单位： | 1. 成都中医药大学,成都,610075
2. 成都中医药大学峨眉学院,四川峨眉山,614201 |
| |
| 基金项目： | 国家科技富民强县专项行动计划试点县及科技项目 (国科发农〔2008〕471号) |
| |
| 摘 要： | 目的:建立竹叶柴胡中黄酮类成分(芦丁、槲皮素、山奈素和异鼠李素)HPLC的含量测定方法,为该药材的开发利用提供科学依据.方法:采用C18柱,流动相为甲醇(A)-0.1％磷酸(B)梯度洗脱,流速0.8 nL·min-1,检测波长360 nm,柱温35℃,进样体积10 μL.结果:芦丁在667.8 ～33 390 ng(r =0.9998),槲皮素在216.0～10 798.4 ng(r =0.9999),山奈素在27.5 ～1372.8 ng(r =0.9999),异鼠李素在130.6～6528.0 ng(r =0.9998)线性关系良好,平均加样回收率分别为100.3％( RSD 0.78％),94.5％ (RSD 1.13％),94.5％( RSD 1.15％),94.1％ (RSD 1.09％).结论:该法在同一色谱系统中同时测定芦丁、槲皮素、山奈素和异鼠李素的含量,操作简便、准确性高、重复性好,用于竹叶柴胡药材的质量评价.
|
| 关 键 词： | 竹叶柴胡 黄酮 高效液相色谱法 |
| 收稿时间： | 2011-12-19 |
Determination of Flavonoid of Bupleurum marginatum by HPLC |
| |
| LIN Hai-xi,WANG Shu-lin,WANG Yan and SUN Cui-ping. Determination of Flavonoid of Bupleurum marginatum by HPLC[J]. China Journal of Experimental Traditional Medical Formulae, 2012, 18(15): 76-79 |
| |
| Authors: | LIN Hai-xi WANG Shu-lin WANG Yan SUN Cui-ping |
| |
| Affiliation: | Chengdu University of Traditional Chinese Medicine, Chengdu 610075,China;Institute of Emei,Chengdu University of Traditional Chinese Medicine, Emei 614201,China;Chengdu University of Traditional Chinese Medicine, Chengdu 610075,China;Chengdu University of Traditional Chinese Medicine, Chengdu 610075,China |
| |
| Abstract: | Objective:To develop a method by HPLC for determination of flavonoid of Bupleurum marginatum.Method: Agilent Zorbax SB C18 column(4.60 mm×150 mm,5 μm),mobile phase methanol-0.1% phosphoric gradient elution,flow rate 0.8 min·mL-1,detected wavelength 360 nm.Result:The linear ranges of rutin,quercetin,kaempferol and isorhamnetin were 667.8-33 390 ng(r=0.999 8),216.0-10 798.4 ng(r=0.999 9),27.5-1 372.8 ng(r=0.999 9),and 130.6-6 528.0 ng(r=0.999 8),the average recovery rates were 100.3%(RSD 0.78%),94.5%(RSD 1.13%),94.5%(RSD 1.15%) and 94.1%(RSD 1.09%).Conclusion:The HPLC method is simply and rapid,and suitable to assess qualitative of B.marginatum. |
| |
| Keywords: | Bupleurum marginatum flavonoid HPLC |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国实验方剂学杂志》浏览原始摘要信息 |
|
点击此处可从《中国实验方剂学杂志》下载全文 |
|
