苦瓜提取物抑制蛋白质的非酶糖基化

目的:研究苦瓜提取物对蛋白质非酶糖基化终末产物( AGE)生成的抑制作用.方法:体外蛋白质非酶糖基化反应系统中分别加入不同质量浓度的苦瓜提取物(0.01,0.1,1.0 g·L-1)或氮基胍(4 mmol·L-1),在不同时间分别测定蛋白质非酶糖化终末产物的生成量.制备糖尿病小鼠模型,以苦瓜提取物10,30 g·kg-1两个剂量连续ig 14 d,测定苦瓜提取物对糖尿病小鼠血糖以及心、肝、肾组织中AGE含量、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量的影响.结果:在体外系统中,蛋白糖化产物的生成与孵育时间呈正相关,苦瓜提取物浓度依赖性抑制蛋白糖化终末产物的生成(P＜0.01).动物实验表明苦瓜提取物能抑制血糖升高(P ＜0.05,P＜0.01),改善糖尿病小鼠心、肝、肾组织中AGE含量(P ＜0.05,P＜0.01)、升高SOD活性(P ＜0.05,P＜0.01)和降低MDA含量(P＜0.05,P＜0.01),呈现剂量效果正相关.结论:苦瓜提取物体内体外均可有效抑制蛋白糖化终末产物的生成.

Inhibitive Effects of Bitter Melon Extract on the Protein Non-enzymatic Glycation

Objective: Using in vitro and in vivo models, the role of bitter melon extract on protein non-enzymatic glycation were investigated. Method : In vitro model, different doses of the extract (0.01,0.1,1.0 g·L^-1) or aminogunidine(4 mmol·L^-1) were added, non-enzymatic glycation end products were measured at different time points by fluorescent measurer. In vivo model, diabetes mice were fed with different doses of extract (10, 30 g·kg^-1). The effects of bitter melon extract on blood glucose, advanced glycation end products(AGEs) levels, superoxide dismutases (SOD) activity and maleic dialdehyde (MDA) content in heart, liver and kidney tissue were assayed. Result : In vitro system, protein glycation products formation was positively correlated with the incubation time, bitter melon extract inhibited the formation of advanced glycation end products dose-dependently (P <0.01). Animal experiments showed that bitter melon extract inhibited blood sugar of diabetic mice(P <0.05, P <0.01). AGE levels and MDA content of heart, liver and kidney tissue were lowed significantly in group which fed with extract (P <0.05, P <0.01). Also, SOD activity was enhanced in group which fed with extract (P <0.05, P <0.01). Conclusion : Bitter melon extract have inhibitive effects on the formation of advanced glycation end products in vitro and in vivo.